Diversity of acidophilic prokaryotes at two acid mine drainage sites in Turkey
- 478 Downloads
The biodiversity of acidophilic prokaryotes in two acidic (pH 2.8–3.05) mine drainage (AMD) sites (Balya and Çan) in Turkey was examined using a combined cultivation-based and cultivation-independent approach. The latter included analyzing microbial diversity using fluorescent in situ hybridization (FISH), terminal restriction enzyme fragment length polymorphism (`T-RFLP), and quantitative PCR (qPCR). Numbers of cultivatable heterotrophic acidophilic bacteria were over an order of magnitude greater than those of chemolithotrophic acidophiles in both AMD ponds examined. Isolates identified as strains of Acidithiobacillus ferrivorans, Acidiphilium organovorum, and Ferrimicrobium acidiphilum were isolated from the Balya AMD pond, and others identified as strains of Leptospirillum ferriphilum, Acidicapsa ligni, and Acidiphilium rubrum from Çan AMD. Other isolates were too distantly related (from analysis of their 16S rRNA genes) to be identified at the species level. Archaeal diversity in the two ponds appeared to be far more limited. T-RFLP and qPCR confirmed the presence of Ferroplasma-like prokaryotes, but no archaea were isolated from the two sites. qPCR generated semiquantitative data for genera of some of the iron-oxidizing acidophiles isolated and/or detected, suggesting the order of abundance was Leptospirillum > Ferroplasma > Acidithiobacillus (Balya AMD) and Ferroplasma > Leptospirillum > Acidithiobacillus (Çan AMD).
KeywordsAcidophile Acidic mine drainage Microbial diversity Turkey
This study is based partly on the PhD thesis of P. Aytar. The study was supported by Eskisehir Osmangazi University Scientific Research Projects Committee (Project No.: 201119018).
- Akyol Z (2012) Balıkesir-Balya cevherli sahalarının jeolojisi, mineralojisi ve maden potansiyelinin değerlendirilmesi. İstanbul Yerbilimleri Dergisi 3:1–2Google Scholar
- Blowes DW, Ptacek CJ, Jambor JL, Weisener CG, Paktunc, D, Gould, WD, Johnson DB (2013) The geochemistry of acid mine drainage. In: Treatise on Geochemistry, 2nd Edition, ElsevierGoogle Scholar
- Dereeper A, Guignon V, Blanc G, Audic S, Buffet S et al (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 1:36 (Web Server Issue): W465–9.0Google Scholar
- Edwards KJ, Gihring TM, Banfield JF (1999) Seasonal variations in microbial populations and environmental conditions in an extreme acid mine environment. Appl Environ Microbiol 65:3627–3632Google Scholar
- Hallberg KB, Johnson DB (2007) Isolation, enumeration, growth, and preservation of acidophilic prokaryotes. In: Hurst CJ, Crawford RL, Garland JL, Lipson DA, Mills AL, Stetzenbach LD (eds) Manual of Environmental Microbiology, 3rd edn. ASM Press, Washington, DC, pp 1155–1165Google Scholar
- Kay CM, Rowe OF, Rocchetti L, Coupland K, Hallberg KB, Johnson DB (2013) Evolution of microbial “streamer” growths in an acidic metal-contaminated stream draining an abandoned underground copper mine. Life doi: 10.3390/life3010189#_blank 3:189-211
- Kulichevskaya IS, Kostina LA, Valášková V, Rijpstra WIC, Damsté JSS, de Boer W, Svetlana N, Dedysh SN (2012) Acidicapsa borealis gen. nov., sp. nov. and Acidicapsa ligni sp. nov., subdivision 1 Acidobacteria from Sphagnum peat and decaying wood. Int J Syst Evol Microbiol 62:1512–1520CrossRefGoogle Scholar
- Lopez-Archilla AI, Marín I, Amils R (2001) Microbial community composition and ecology of an acidic aquatic environment: the Tinto River, Spain. Microbial Ecol 41:20–35Google Scholar
- Snaidr MO, Amann R, Huber I, Ludwig W, Schleifer KH (1997) Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl Environ Microbiol 63:2884–2896Google Scholar