Environmental Science and Pollution Research

, Volume 22, Issue 4, pp 2599–2609 | Cite as

Integrated treatment of municipal sewage sludge by deep dewatering and anaerobic fermentation for biohydrogenproduction

  • Li Yu
  • Yang Yu
  • Wentian Jiang
  • Huangzhao Wei
  • Chenglin SunEmail author
Research Article


The increasing sludge generated in wastewater treatment plants poses a threat to the environment. Based on the traditional processes, sludge dewatered by usual methods was further dewatered by hydraulic compression and the filtrate released was treated by anaerobic fermentation. The difficulties in sludge dewatering were associated with the existence of sludge flocs or colloidal materials. A suitable CaO dosage of 125 mg/g dry sludge (DS) could further decrease the moisture content of sludge from 82.4 to 50.9 %. The filtrate from the dewatering procedure was a potential substrate for biohydrogen production. Adding zero-valent iron (ZVI) into the anaerobic system improved the biohydrogen yield by 20 %, and the COD removal rate was lifted by 10 % as well. Meanwhile, the sludge morphology and microbial community were altered. The novel method could greatly reduce the sludge volume and successfully treated filtrate along with the conversion of organics into biohydrogen.


Sewage sludge Calcium oxide Dewatering Biohydrogen Fermentation Zero-valent iron (ZVI) 


  1. American Public Health Association (1998) Standard methods for the examination of water and wastewater, 20th edn. APHA, Washington, DCGoogle Scholar
  2. Arjoon A, Olaniran AO, Pillay B (2013) Co-contamination of water with chlorinated hydrocarbons and heavy metals: challenges and current bioremediation strategies. Int J Environ Sci Technol 10(2):395–412CrossRefGoogle Scholar
  3. Cai ML, Liu JX, Wei YS (2004) Enhanced biohydrogen production from sewage sludge with alkaline pretreatment. Environ Sci Technol 38(11):3195–3202CrossRefGoogle Scholar
  4. Chakkrit S, Pensri PK, Tsuyoshi I, Alissara RS (2011) Co-digestion of food waste and sludge for hydrogen production by anaerobic mixed cultures: statistical dey factors optimization. Int J Hydrog Energy 36(21):14227–14237CrossRefGoogle Scholar
  5. Chang FY, Lin CY (2006) Calcium effect on fermentative hydrogen production in an anaerobic up-flow sludge blanket system. Water Sci Technol 54(9):105–112CrossRefGoogle Scholar
  6. Chang MH, Shu HY, Yu HH, Sung YC (2006) Reductive decolourization and total organic carbon reduction of the diazo dye CI acid black 24 by zero-valent iron powder. J Chem Technol Biotechnol 81:1259–1266CrossRefGoogle Scholar
  7. Chung J, Lee M, Ahn J, Bae W, Lee YW, Shim H (2009) Effects of operational conditions on sludge degradation and organic acids formation in low-critical wet air oxidation. J Hazard Mater 162:10–16CrossRefGoogle Scholar
  8. Davidson EA, Giezen M, Horner DS, Embley TM, Howe CJ (2002) An [Fe] hydrogenase from the anaerobic hydrogenosome-containing fungus Neocallimastix frontalis L2. Gene 295:45–52CrossRefGoogle Scholar
  9. Das A, Frank CJ (2000) Adhesion of the dissimilatory Fe (III)-reducing bacteria Shewnella alga BrY to crystalline Fe (III) oxides. Curr Microbiol 42:151–154CrossRefGoogle Scholar
  10. Feng YH, Zhang YB, Quan X, Chen S (2014) Enhanced anaerobic digestion of waste activated sludge digestion by the addition of zero valent iron. Water Res 52:242–250CrossRefGoogle Scholar
  11. Fux C, Velten S, Carozzi V, Solley D, Keller J (2006) Efficient and stable nitritation and denitritation of ammonium-rich sludge dewatering liquor using an SBR with continuous loading. Water Res 40:2765–2775CrossRefGoogle Scholar
  12. Fytili D, Zabaniotou A (2008) Utilization of sewage sludge in EU application of old and new methods—a review. Renew Sust Energ Rev 12:116–140CrossRefGoogle Scholar
  13. Guan BH, Yu J, Fu HL, Guo MH, Xu XH (2012) Improvement of activated sludge dewaterability by mild thermal treatment in CaCl2 solution. Water Res 46:425–432CrossRefGoogle Scholar
  14. Hay JXW, Wu TY, Juan JC, Jahim JM (2013) Biohydrogen production through photo fermentation or dark fermentation using waste as a substrate: overview, economics, and future prospects of hydrogen usage. Biofuel Bioprod Bior 7(3):334–352CrossRefGoogle Scholar
  15. Higgins MJ, Novak JT (1997) The effect of cations on the settling and dewatering of activated sludges: laboratory results. Water Environ Res 69(2):215–224CrossRefGoogle Scholar
  16. Ho KL, Lee DJ, Su A, Chang JS (2012) Biohydrogen from cellulosic feedstock: dilution-to-stimulation approach. Int J Hydrog Energy 37:15582–15587CrossRefGoogle Scholar
  17. Jung KW, Kim DH, Kim SH, Shin HS (2011) Bioreactor design for continuous dark fermentative hydrogen production. Bioresour Technol 102:8612–8620CrossRefGoogle Scholar
  18. Kalloum S, Bouabdessalem H, Touzi A, Iddou A, Ouali MS (2011) Biogas production from the sludge of the municipal wastewater treatment plant of Adrar city (southwest of Algeria). Biomass Bioenergy 35:2554–2560CrossRefGoogle Scholar
  19. Kang JH, Kim D, Lee TJ (2012) Hydrogen production and microbial diversity in sewage sludge fermentation preceded by heat and alkaline treatment. Bioresour Technol 109:239–243CrossRefGoogle Scholar
  20. Kelessidis A, Stasinakis AS (2012) Comparative study of the methods used for treatment and final disposal of sewage sludge in European countries. Waste Manag 32:1186–1195CrossRefGoogle Scholar
  21. Kim KH, Kim SH, Kim HW, Kim MS, Shin HS (2011) Sewage sludge addition to food waste synergistically enhances hydrogen fermentation performance. Bioresour Technol 102:8501–8506CrossRefGoogle Scholar
  22. Li CL, Fang HHP (2007) Fermentative hydrogen production from wastewater and solid wastes by mixed cultures. Crit Rev Environ Sci Technol 37:1–39CrossRefGoogle Scholar
  23. Li WW, Zhang Y, Zhao JB, Yang YL, Zeng RJ, Liu HQ, Feng YJ (2013) Synergetic decolorization of reactive blue 13 by zero-valent iron and anaerobic sludge. Bioresour Technol 149:38–43CrossRefGoogle Scholar
  24. Li YL, Liu JW, Chen JY, Shi YF, Mao W, Liu H, Li Y, He S, Yang JK (2014) Reuse of dewatered sewage sludge conditioned with skeleton builders as landfill cover material. Int J Environ Sci Technol 11(1):233–240CrossRefGoogle Scholar
  25. Liao BQ, Allen DG, Droppo IG, Leppard GG, Liss SN (2000) Bound water content of activated sludge and its relationship to solids retention time, floc structure and surface properties. Water Environ Res 72:722–730CrossRefGoogle Scholar
  26. Meng XS, Zhang YB, Li Q, Quan X (2013) Adding Fe0 powder to enhance the anaerobic conversion of propionate to acetate. Biochem Eng J 73:80–85CrossRefGoogle Scholar
  27. Mihoubi D (2004) Mechanical and thermal dewatering of residual sludge. Desalination 167:135–139CrossRefGoogle Scholar
  28. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–427CrossRefGoogle Scholar
  29. Mohan SV, Babu VL, Sarma PN (2008) Effect of various pretreatment methods on anaerobic mixed microflora to enhance biohydrogen production utilizing dairy wastewater as substrate. Bioresour Technol 99:59–67CrossRefGoogle Scholar
  30. Mudhoo A, Kumar S (2013) Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass. Int J Environ Sci Technol 10(6):1383–1398CrossRefGoogle Scholar
  31. Mustin SD, Lartiges BS, Villemin G, Thomas F, Yvon J, Bersillon JL, Snidaro D (2001) Ferric chloride and lime conditioning of activated sludges: an electron microscopic study on resin-embedded samples. Water Res 35:3018–3024CrossRefGoogle Scholar
  32. Ning YY, Wang SF, Jin DW, Harada H, Shi XY (2013) Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor. Renew Energy 53:12–17CrossRefGoogle Scholar
  33. Nishiyama T, Ueki A, Kaku N, Watanabe K, Ueki K (2009) Bacteroides graminisolvens sp nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste. Int J Syst Evol Microbiol 59:1901–1907CrossRefGoogle Scholar
  34. Pevere A, Guibaud G, Hullebusch ED, Boughzala W, Lens PNL (2007) Effect of Na+ and Ca2+ on the aggregation properties of sieved anaerobic granular sludge. Colloid Surf A 306:142–149CrossRefGoogle Scholar
  35. Qi Y, Thapa KB, Hoadley AFA (2011) Application of filtration aids for improving sludge dewatering properties—a review. Chem Eng J 171:373–384CrossRefGoogle Scholar
  36. Ren NQ, Zhao D, Chen XL (2001) Control of fermentation types in continuous-flow acidogenic reactors: effects of pH and redox potential. J Harbin I Technol 8:116–119Google Scholar
  37. Shanmugam SR, Chaganti SR, Lalman JA, Heath DD (2014) Effect of inhibitors on hydrogen consumption and microbial population dynamics in mixed anaerobic cultures. Int J Hydrog Energy 39:249–257CrossRefGoogle Scholar
  38. Shih YH, Chou HL, Peng YH, Chang CY (2012) Synergistic effect of microscale zerovalent iron particles combined with anaerobic sludges on the degradation of decabromodiphenyl ether. Bioresour Technol 108:14–20CrossRefGoogle Scholar
  39. Wakeman RJ (2007) Separation technologies for sludge dewatering. J Hazard Mater 144:614–619CrossRefGoogle Scholar
  40. Wei YT, Wu SC, Yang SW, Che CH, Lien HL, Huang DH (2012) Biodegradable surfactant stabilized nanoscale zero-valent iron for in situ treatment of vinyl chloride and 1,2-dichloroethane. J Hazard Mater 211–212:373–380CrossRefGoogle Scholar
  41. Wu TY, Hay JXW, Kong LB, Juan JC, Jahim JM (2012) Recent advances in reuse of waste material as substrate to produce biohydrogen by purple non-sulfur (PNS) bacteria. Renew Sust Energ Rev 16(5):3117–3122CrossRefGoogle Scholar
  42. Xiao BY, Liu JX (2009) Biological hydrogen production from sterilized sewage sludge by anaerobic self-fermentation. J Hazard Mater 168:163–167CrossRefGoogle Scholar
  43. Yin X, Han PF, Lu XP, Wang YR (2004) A review on the dewaterability of bio-sludge and ultrasound pretreatment. Ultrason Sonochem 11:337–348Google Scholar
  44. Yu XY, Zhang ST, Xu H, Zheng L, Lü XB, Ma DG (2010) Influence of filter cloth on the cathode on the electroosmotic dewatering of activated sludge. Chin J Chem Eng 18:562–568CrossRefGoogle Scholar
  45. Zhai LF, Sun M, Song W, Wang G (2012) An integrated approach to optimize the conditioning chemicals for enhanced sludge conditioning in a pilot-scale sludge dewatering process. Bioresour Technol 121:161–168CrossRefGoogle Scholar
  46. Zhang YB, An AL, Quan X (2011) Enhancement of sludge granulation in a zero valence iron packed anaerobic reactor with a hydraulic circulation. Process Biochem 46:471–476CrossRefGoogle Scholar
  47. Zhu HG, Seto P, Parker WJ (2014) Enhanced dark fermentative hydrogen production under the effect of zero-valent iron shavings. Int J Hydrogen Energy. doi: 10.1016/j.ijhydene.2014.06.055 Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Li Yu
    • 1
    • 2
  • Yang Yu
    • 1
    • 2
  • Wentian Jiang
    • 1
  • Huangzhao Wei
    • 1
  • Chenglin Sun
    • 1
    Email author
  1. 1.Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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