Environmental Science and Pollution Research

, Volume 22, Issue 2, pp 988–995

Soil genotoxicity assessment—results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization

  • Sylvie Cotelle
  • Adrien Dhyèvre
  • Serge Muller
  • Pascale Chenon
  • Nicolas Manier
  • Pascal Pandard
  • Abdelwahad Echairi
  • Jérôme Silvestre
  • Maritxu Guiresse
  • Eric Pinelli
  • Lucia Giorgetti
  • Meri Barbafieri
  • Valéria C. Silva
  • Fernanda Engel
  • Claudemir M. Radetski
Research Article

Abstract

The Vicia micronucleus assay was standardized in an international protocol, ISO 29200, “Assessment of genotoxic effects on higher plants—Vicia faba micronucleus test,” for soil or soil materials (e.g., compost, sludge, sediment, waste, and fertilizing materials). The aim of this interlaboratory study on the Vicia micronucleus assay was to investigate the robustness of this in vivo assay in terms of its applicability in different countries where each participant were asked to use their own seeds and reference soil, in agreement with the ISO 29200 standard. The ISO 29200 standard protocol was adopted for this study, and seven laboratories from three countries (France, Italy, and Brazil) participated in the study. Negative and positive controls were correctly evaluated by 100 % of the participants. In the solid-phase test, the micronucleus frequency (number of micronuclei/1,000 cells) varied from 0.0 to 1.8 for the negative control (i.e., Hoagland’s solution) and from 5.8 to 85.7 for the positive control (i.e., maleic hydrazide), while these values varied from 0.0 to 1.7 for the negative control and from 14.3 to 97.7 for the positive control in the case of liquid-phase test. The variability in the data obtained does not adversely affect the robustness of the protocol assessed, on the condition that the methodology described in the standard ISO 29200 is strictly respected. Thus, the Vicia micronucleus test (ISO 29200) is appropriate for complementing prokaryotic or in vitro tests cited in legislation related to risk assessment of genotoxicity potential.

Keywords

Vicia faba Genotoxicity Micronucleus assay Interlaboratory study Contaminated soil ISO 29200 

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Sylvie Cotelle
    • 1
  • Adrien Dhyèvre
    • 1
  • Serge Muller
    • 1
  • Pascale Chenon
    • 2
  • Nicolas Manier
    • 3
  • Pascal Pandard
    • 3
  • Abdelwahad Echairi
    • 4
  • Jérôme Silvestre
    • 5
    • 6
  • Maritxu Guiresse
    • 5
    • 6
  • Eric Pinelli
    • 5
    • 6
  • Lucia Giorgetti
    • 7
  • Meri Barbafieri
    • 8
  • Valéria C. Silva
    • 9
  • Fernanda Engel
    • 9
  • Claudemir M. Radetski
    • 9
  1. 1.Laboratoire Interdisciplinaire des Environnements ContinentauxUniversité de Lorraine (LIEC), CNRS UMR 7360MetzFrance
  2. 2.RITTMO Agro-EnvironnementColmarFrance
  3. 3.INERIS, Parc technologique AlataBP2 Verneuil-en-HalatteFrance
  4. 4.WELIENCE Agro-EnvironnementRD 31, BretenièreFrance
  5. 5.Université de Toulouse; INP, UPSEcoLab (Laboratoire d’Ecologie Fonctionnelle et Environnement ENSATCastanet TolosanFrance
  6. 6.CNRS EcoLabCastanet TolosanFrance
  7. 7.National Research Council (CNR), Institute of Biology and Agricultural Biotechnology (IBBA)Research Unit of PisaPisaItaly
  8. 8.National Research Council (CNR), Institute of Ecosystem Study (ISE)Research Unit of PisaPisaItaly
  9. 9.UNIVALI-Universidade do Vale do ItajaíLaboratório de Remediação AmbientalItajaíBrazil

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