Environmental Science and Pollution Research

, Volume 21, Issue 24, pp 13720–13731 | Cite as

Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout

  • Florane Le Bihanic
  • Bénédicte Morin
  • Xavier Cousin
  • Karyn Le Menach
  • Hélène Budzinski
  • Jérôme Cachot
PAHs and fish – Exposure monitoring and adverse effects – from molecular to individual level

Abstract

A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic extract from a PAH-contaminated sediment (Seine estuary, France) and two petrogenic extracts from Arabian Light and Erika oils, at two environmental concentrations, 3 and 10 μg g−1 sum of PAHs. The degree and spectrum of toxicity were different according to the extract considered. Acute effects including embryo mortality and decreased hatching success were observed only for Erika oil extract. Arabian Light and pyrolytic extracts induced mainly sublethal effects including reduced larvae size and hemorrhages. Arabian Light and Erika extracts both induced repairable DNA damage as revealed by the comet assay versus the micronucleus assay. The concentration and proportion of methylphenanthrenes and methylanthracenes appeared to drive the toxicity of the three PAH fractions tested, featuring a toxic gradient as follows: pyrolytic < Arabian Light < Erika. The minimal concentration causing developmental defects was as low as 0.7 μg g−1 sum of PAHs, indicating the high sensitivity of the assay and validating its use for toxicity assessment of particle-bound pollutants.

Keywords

PAH mixture Oil extract Pyrolytic extract Embryotoxicity Developmental defects Genotoxicity 

Abbreviations

PAH

Polycyclic aromatic hydrocarbon

AhR

Aryl hydrocarbon receptor

DD

Degree day

dw

Dry weight

BaP

Benzo[a]pyrene

Fluo

Fluoranthene

ELS

Early life stage

MN

Micronucleus

PY

Pyrolytic

LO

Arabian Light oil

HO

Erika heavy oil

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Florane Le Bihanic
    • 1
  • Bénédicte Morin
    • 1
  • Xavier Cousin
    • 2
    • 3
  • Karyn Le Menach
    • 1
  • Hélène Budzinski
    • 1
  • Jérôme Cachot
    • 1
  1. 1.University of Bordeaux, EPOC, UMR CNRS 5805Talence CedexFrance
  2. 2.IFREMER, Ecotoxicology laboratoryL’HoumeauFrance
  3. 3.INRA LPGP, Campus de BeaulieuRennesFrance

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