Environmental Science and Pollution Research

, Volume 21, Issue 13, pp 8084–8094 | Cite as

Mammalian cell line-based bioassays for toxicological evaluation of landfill leachate treated by Pseudomonas sp. ISTDF1

  • Pooja Ghosh
  • Mihir Tanay Das
  • Indu Shekhar Thakur
Research Article


Landfill leachate has become a serious environmental concern because of the presence of many hazardous compounds which even at trace levels are a threat to human health and environment. Therefore, it is important to assess the toxicity of leachate generated and discharge it conforming to the safety standards. The present work examined the efficiency of an earlier reported Pseudomonas sp. strain ISTDF1 for detoxification of leachate collected from Okhla landfill site (New Delhi, India). GC-MS analysis performed after treatment showed the removal of compounds like alpha-limonene diepoxide, brominated dioxin-2-one, Bisphenol A, nitromusk, phthalate derivative, and nitrobenzene originally found in untreated leachate. ICP-AES analysis for heavy metals also showed reduction in concentrations of Zn, Cd, Cr, Fe, Ni, and Pb bringing them within the limit of safety discharge. Methyl tetrazolium (MTT) assay for cytotoxicity, alkaline comet assay for genotoxicity, and 7-ethoxyresorufin-O-deethylase (EROD) assay for dioxin-like behavior were carried out in human hepato-carcinoma cell line HepG2 to evaluate the toxic potential of treated and untreated leachates. The bacterium reduced toxicity as shown by 2.5-fold reduction of MTT EC50 value, 7-fold reduction in Olive Tail Moment, and 2.8-fold reduction in EROD induction after 240 h of bacterial treatment.


Landfill leachate Bioremediation Pseudomonas sp. Toxicity reduction HepG2 cell line 



This work was supported by research grant from Council of Scientific and Industrial Research (CSIR), New Delhi, India. We thank Dr. J.K Tripathi (SES, JNU, New Delhi) for ICP-AES analysis and Mr. Ajai Kumar (Advanced Instrumentation Research Facility-AIRF, JNU, New Delhi) for GC-MS analysis.

Supplementary material

11356_2014_2802_MOESM1_ESM.doc (24.4 mb)
ESM 1 (DOC 24937 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Pooja Ghosh
    • 1
  • Mihir Tanay Das
    • 1
  • Indu Shekhar Thakur
    • 1
  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia

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