Environmental Science and Pollution Research

, Volume 21, Issue 9, pp 5803–5809 | Cite as

Sensitive time-resolved fluoroimmunoassay for quantitative determination of clothianidin in agricultural samples

  • Ming Li
  • Enze Sheng
  • Yulong Yuan
  • Xiaofeng Liu
  • Xiude Hua
  • Minghua Wang
Research Article
First Online:
Received:
Accepted:

Abstract

Europium (Eu3+)-labeled antibody was used as a fluorescent label to develop a highly sensitive time-resolved fluoroimmunoassay (TRFIA) for determination of clothianidin residues in agricultural samples. Toward this goal, the Eu3+-labeled polyclonal antibody and goat anti-rabbit antibody were prepared for developing and evaluating direct competitive TRFIA (dc-TRFIA) and indirect competitive TRFIA (ic-TRFIA). Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of clothianidin were 9.20 and 0.0909 μg/L for the dc-TRFIA and 2.07 and 0.0220 μg/L for the ic-TRFIA, respectively. The ic-TRFIA has no obvious cross-reactivity with the analogues of clothianidin except for dinotefuran. The average recoveries of clothianidin from spiked water, soil, cabbage, and rice samples were estimated to range from 74.1 to 115.9 %, with relative standard deviations of 3.3 to 11.7 %. The results of TRFIA for the blind samples were largely consistent with gas chromatography (R 2 = 0.9902). The optimized ic-TRFIA might become a sensitive and satisfactory analytical method for the quantitative monitoring of clothianidin residues in agricultural samples.

Keywords

Clothianidin Europium Polyclonal antibody Time-resolved fluoroimmunoassay 
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Notes

Acknowledgments

This work was supported by the National “863” High-Tech Research Program of China (no. 2011AA100806), the Special Fund for Agro-scientific Research in the Public Interest (no. 201203022), and the Project of Graduates’ Research Innovative Programs from Colleges and Universities of Jiangsu Province (no. CXZZ13-0305).

References

  1. Chen MF, Huang JW, Wong SS, Li GC (2005) Analysis of insecticide clothianidin and its metabolites in rice by liquid chromatography with a UV detector. J Food Drug Anal 13:279–283Google Scholar
  2. Chen YJ, Wang Y, Li N, Gong ML (2009) Methodological study on the determination of residual pesticide in Brassica chinensis by HPLC. China Trop Med 9:361–362Google Scholar
  3. Esteve-Turrillas FA, Parra J, Abad-Fuentes A, Agullo C, Abad-Somovilla A, Mercader JV (2010) Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer. Anal Chim Acta 682:93–103CrossRefGoogle Scholar
  4. Gui WJ, Jin MJ, Sun LF, Guo YR, Zhu GN (2009) Residues determination of carbofuran in vegetables based on sensitive time-resolved fluorescence immunoassay. Food Agric Immunol 20:49–56CrossRefGoogle Scholar
  5. Hagan AK, Zuchner T (2011) Lanthanide-based time-resolved luminescence immunoassays. Anal Bioanal Chem 400:2847–2864CrossRefGoogle Scholar
  6. Hou YY, Bian HZ, Zhao XX, Hu YF, Su T, Wang XH, Wan XC (2011) Determination of nicotinoid residues in complicated matrix vegetables by solid phase extraction and HPLC method. J Instrum Anal 30:58–63Google Scholar
  7. Huang B, Zhao LL, Sun JM, Zhang J, Zhang Y, Zhang K, Jin J, Xie MH (2012) Establishment and validation of a time-resolved fluoroimmunoassay for chlorpromazine. Food Anal Methods 5:625–630CrossRefGoogle Scholar
  8. Kim BM, Park JS, Choi JH, El-Aty AM, Na TW, Shim JH (2012) Residual determination of clothianidin and its metabolites in three minor crops via tandem mass spectrometry. Food Chem 131:1546–1551CrossRefGoogle Scholar
  9. Kim MJ, Lee HS, Chung DH, Lee YT (2003) Synthesis of haptens of organophosphorus pesticide and development of enzyme-linked immunosorbent assays for parathion-methyl. Anal Chim Acta 493:47–62CrossRefGoogle Scholar
  10. Li L, Jiang GG, Liu CY, Liang HW, Sun DL, Li W (2012) Clothianidin dissipation in tomato and soil, and distribution in tomato peel and flesh. Food Control 25:265–269CrossRefGoogle Scholar
  11. Li M, Sheng EZ, Cong LJ, Wang MH (2013) Development of immunoassays for detecting clothianidin residue in agricultural products. J Agric Food Chem 61:3919–3623CrossRefGoogle Scholar
  12. Liu YH, Wang CM, Gui WJ, Bi JC, Jin MJ, Zhu GN (2009) Development of a sensitive competitive indirect ELISA for parathion residue in agricultural and environmental samples. Ecotox Environ Safe 72:1673–1679CrossRefGoogle Scholar
  13. Liu ZJ, Yu PM, Fang S, Fan JQ, Wang MH (2011) Development of an enzyme-linked immunosorbent assay for determination of pretilachlor in water and soil. Ecotox Environ Safe 74:1595–1599CrossRefGoogle Scholar
  14. Motohiro T, John YC (2005) Neonicotinoid insecticide toxicology: mechanisms of selective action. Annu Rev Pharmacol Toxicol 45:247–268CrossRefGoogle Scholar
  15. Sesay AM, Micheli L, Tervo P, Palleschi G, Virtanen V (2013) Development of a competitive immunoassay for the determination of cortisol in human saliva. Anal Biochem 434:308–314CrossRefGoogle Scholar
  16. Uchigashima M, Watanabe E, Ito S, Iwasa S, Miyake S (2012) Development of immunoassay based on monoclonal antibody reacted with the neonicotinoid insecticides clothianidin and dinotefuran. Sensors 12:15858–15872CrossRefGoogle Scholar
  17. Uneme H (2011) Chemistry of clothianidin and related compounds. J Agric Food Chem 59:2932–2937CrossRefGoogle Scholar
  18. Wengatz I, Stoutamire DW, Gee SJ, Hammock BD (1998) Development of an enzyme-linked immunosorbent assay for the detection of the pyrethroid insecticide fenpropathrin. J Agric Food Chem 46:2211–2221CrossRefGoogle Scholar
  19. Xu ZL, Dong JX, Yang JY, Wang H, Jiang YM, Lei HT, Shen YD, Sun YM (2012) Development of a sensitive time-resolved fluoroimmunoassay for organophosphorus pesticides in environmental water samples. Anal Methods 4:3484–3490CrossRefGoogle Scholar
  20. Zhang Z, Liu JF, Feng TT, Yao Y, Gao LH, Jiang GB (2013) Time-resolved fluoroimmunoassay as an advantageous analytical method for assessing the total concentration and environmental risk of fluoroquinolones in surface waters. Environ Sci Technol 47:454–462CrossRefGoogle Scholar
  21. Zhou YL, Xia XH, Xu Y, Ke W, Yang W, Li QG (2012) Application of europium(III) chelates-bonded silica nanoparticle in time-resolved immunofluorometric detection assay for human thyroid stimulating hormone. Anal Chim Acta 722:95–99CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ming Li
    • 1
  • Enze Sheng
    • 1
  • Yulong Yuan
    • 1
  • Xiaofeng Liu
    • 1
  • Xiude Hua
    • 1
  • Minghua Wang
    • 1
  1. 1.Department of Pesticide Science, College of Plant ProtectionNanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of EducationNanjingPeople’s Republic of China

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