Environmental Science and Pollution Research

, Volume 21, Issue 4, pp 2988–3004 | Cite as

Influence of salinity and temperature on the activity of biosurfactants by polychaete-associated isolates

  • Carmen Rizzo
  • Luigi Michaud
  • Christoph Syldatk
  • Rudolf Hausmann
  • Emilio De Domenico
  • Angelina Lo Giudice
Research Article


Influence of different parameters on biosurfactant (BS) activity was carried out on strains that were isolated from the polychaetes Megalomma claparedei, Sabella spallanzanii and Branchiomma luctuosum and additional 30 strains that were previously identified as potential BS producers from crude oil enrichments of the same polychaete specimens. The selection of BS-producing strains from polychaete natural samples was carried out by using standard screening tests. The BS activity by each isolate was evaluated for the effect of salinity and temperature on emulsion production and surface tension reduction, during incubation in mineral medium supplemented with tetradecane or diesel oil. All isolates showed a similar time course of BS activity, and the latter was more influenced by salinity rather than temperature. Some of the BS producers belonged to genera that have not (i.e. Citricoccus, Cellulophaga, Tenacibaculum and Maribacter) or have poorly been (Psychrobacter, Vibrio, and Pseudoalteromonas) reported as able to produce BSs. This is remarkable as some of them have previously been detected in hydrocarbon-enriched samples. Results confirm that filter-feeding polychaetes are an efficient source for the isolation of BS producers.


Biosurfactants Filter-feeding organisms Salinity Temperature Megalomma claparedei Sabella spallanzanii Branchiomma luctuosum 



Rizzo C. wishes to thank all the colleagues at the Karlsruher Institut für Technologie (KIT), Germany, for assistance and support during her stay in their lab. The authors also thank Dr. Andrea Cosentino for the taxonomic identification of Polychaetes. This research was supported by grants from the Doctoral School ‘Dottorato di Ricerca in Scienze Ambientali: Ambiente Marino e Risorse’ of the University of Messina, Italy.

Supplementary material

11356_2013_2259_MOESM1_ESM.doc (68 kb)
Table S1 (DOC 68 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Carmen Rizzo
    • 1
  • Luigi Michaud
    • 1
  • Christoph Syldatk
    • 2
  • Rudolf Hausmann
    • 3
  • Emilio De Domenico
    • 1
  • Angelina Lo Giudice
    • 1
    • 4
  1. 1.Department of Biological and Environmental SciencesUniversity of MessinaMessinaItaly
  2. 2.Institute of Process Engineering in Life Sciences, Section II: Technical BiologyKarlsruhe Institute of Technology (KIT)KarlsruheGermany
  3. 3.Institute of Food Science and Biotechnology, Section Bioprocess EngineeringUniversity of HohenheimStuttgartGermany
  4. 4.Department of Biological and Environmental Sciences (DISBA)Università di MessinaMessinaItaly

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