A new bioassay proposed in the patent P201300029 was applied to a pre-treated wastewater containing a mixture of commercial pesticides to simulate a recalcitrant industrial wastewater in order to determine its biodegradability. The test uses a mixture of standardized inoculum of the lyophilized bacteria Pseudomonas putida with the proper proportion of salts and minerals. The results highlight that biodegradation efficiency can be calculated using a gross parameter (chemical oxygen demand (COD)) which facilitates the biodegradability determination for routine water biodegradability analysis. The same trend was observed throughout the assay with the dehydrated and fresh inoculums, and only a difference of 5 % in biodegradation efficiency (E f) was observed. The obtained results showed that the P. putida biodegradability assay can be used as a commercial test with a lyophilized inoculum in order to monitor the ready biodegradability of an organic pollutant or a WWTP influent. Moreover, a combination of the BOD5/COD ratio and the P. putida biodegradability test is an attractive alternative in order to evaluate the biodegradability enhancement in water pre-treated with advanced oxidation processes (AOPs).
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Amat AM, Arques A, García-Ripoll A, Santos-Juanes L, Vicente R, Oller I, Maldonado MI, Malato S (2009) A reliable monitoring of the biocompatibility of an effluent along an oxidative pre-treatment by sequential bioassays and chemical analyses. Water Res 43:784–792
Ballesteros Martín MM, Sánchez Pérez JA, García Sánchez JL, Casas López JL, Malato S (2009) Effect of pesticide concentration on the degradation process by combined solar photo-Fenton and biological treatment. Water Res 43:3838–3848
Ballesteros Martín MM, Casas López JL, Oller I, Malato S, Sánchez Pérez JA (2010) A comparative study of different tests for biodegradability enhancement determination during AOP treatment of recalcitrant toxic aqueous solutions. Ecotox Environ Saf 73:1189–1195
Ballesteros Martín MM, Casas López JL, Malato Rodríguez S, Sánchez Pérez JA (2013) Patent P201300029. Ensayo y procedimiento para la evaluación de la biodegradabilidad rápida de aguas mediante Pseudomonas putida
Bila DM, Filipe Montalvão A, Silva AC, Dezotti M (2005) Ozonation of a landfill leachate: evaluation of toxicity removal and biodegradability improvement. J Haz Mat 117:235–242
Blok J, Booy M (1984) Biodegradability test results related to quality and quantity of the inoculum. Ecotox Environ Safe 8:410–422
Contreras S, Rodríguez M, Al Momani F, Sans C, Esplugas S (2003) Contribution of the ozonation pre-treatment to the biodegradation of aqueous solutions of 2,4-dichlorophenol. Water Res 37:3164–3171
Fanchiang JM, Tseng DH (2009) Degradation of anthraquinone dye C.I. Reactive Blue 19 in aqueous solution by ozonation. Chemosphere 77:214–221
Farré MJ, Doménech X, Peral J (2006) Assessment of photo-Fenton and biological treatment coupling for diuron and linuron removal from water. Water Res 40:2533–2540
Fitzmaurice GD, Gray NF (1989) Evaluation of manufactured inocula for use in the BOD test. Water Res 23:655–657
Gogate R, Pandit B (2004) A review of imperative technologies for wastewater treatment II: hybrid methods. Adv Environ Res 8:553–597
Kreetachat T, Damrongsri M, Punsuwon V, Vaithanomsat P, Chiemchaisri C, Chomsurin C (2007) Effects of ozonation process on lignin-derived compounds in pulp and paper mill effluents. J Haz Mat 142:250–257
Kümmerer K, Alexy R, Hüttig J, Schöll A (2004) Standardized tests fail to assess the effects of antibiotics on environmental bacteria. Water Res 38:2111–2116
Mantzavinos D, Psillakis E (2004) Enhancement of biodegradability of industrial wastewaters by chemical oxidation pre-treatment. J Chem Technol Biotechnol 79:431–454
Marco A, Esplugas S, Saum G (1997) How and why combine chemical and biological processes for wastewater treatment. Water Sci Technol 35:321–327
Melero JA, Martínez F, Botas JA, Molina R, Pariente MI (2009) Heterogeneous catalytic wet peroxide oxidation systems for the treatment of an industrial pharmaceutical wastewater. Water Res 43:4010–4018
Metcalf, Eddy (1985) Wastewater engineering: treatment, disposal and reuse, 3rd edn. McGraw-Hill, New York
OECD (2005) OECD guideline for testing of chemicals. Proposal for revised introduction to the OECD guidelines for testing of chemicals, Section 3. ENV/JM/TG(2005)5/REV1. OECD, Paris
Oller I, Gernjak W, Maldonado MI, Pérez-Estrada LA, Sánchez-Pérez JA, Malato S (2006) Solar photocatalytic degradation of some hazardous water-soluble pesticides at pilot-plant scale. J Hazard Mater 138:507–517
Oller I, Malato S, Sánchez-Pérez JA (2011) Combination of advanced oxidation processes and biological treatments for wastewater decontamination—a review. Sci Total Environ 409:4141–4166
Paixao SM, Baeta-Hall L, Anselmo AM (2000) Evaluation of two commercial microbial inocula as seed in a 5-day biochemical oxygen demand test. Water Environ Res 72:282–284
Sarria V, Parra S, Adler N, Péringer P, Benitez N, Pulgarin C (2002) Recent developments in the coupling of photoassisted and aerobic biological processes for the treatment of biorecalcitrant compounds. Catal Today 76:301–315
Shim H, Shin E, Yang ST (2002) A continuous fibrous-bed bioreactor for BTEX biodegradation by a co-culture of Pseudomonas putida and Pseudomonas fluorescens. Adv Environ Res 7:203–216
Tišler T, Jemec A, Mozetič B, Trebše P (2009) Hazard identification of imidacloprid to aquatic environment. Chemosphere 76:907–914
Van Ginkel CG, Haan A, Luijten MLGC, Stroo CA (1995) Influence of the size and source of the inoculum on biodegradation curves in closed-bottle tests. Ecotox Environ Saf 31:218–223
Vázquez-Rodríguez GA, Calmon A, Silvestre F, Goma G, Rols JL (2006) Effect of the inoculation level in aerobic biodegradability tests of polymeric materials. Int Biodeter Biodeg 58:44–47
Vilar VJP, Capelo SMS, Silva TFCV, Boaventura RAR (2011) Solar photo-Fenton as a pre-oxidation step for biological treatment of landfill leachate in a pilot plant with CPCs. Catal Today 161:228–234
Zapata A, Malato S, Sánchez-Pérez JA, Oller I, Maldonado MI (2010) Scale-up strategy for a combined solar photo-Fenton/biological system for remediation of pesticide-contaminated water. Catal Today 151:100–106
This research was supported by the Consejería de Innovación, Ciencia y Empresa de la Junta de Andalucía (Andalusian Regional Government) (FOTOMEM project, P08-RNM-03772). Elisabet Ortega Gómez would like to acknowledge Ministerio de Economía y Competitividad for her F.P.I. scholarship (Ref: BES-2011-043886).
Responsible editor: Robert Duran
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Ballesteros Martín, M.M., Esteban García, B., Ortega-Gómez, E. et al. A new bioseed for determination of wastewater biodegradability: analysis of the experimental procedure. Environ Sci Pollut Res 21, 9522–9528 (2014). https://doi.org/10.1007/s11356-013-2227-3
- Pseudomonas putida