Environmental Science and Pollution Research

, Volume 21, Issue 2, pp 1064–1070 | Cite as

Elevated water temperature reduces the acute toxicity of the widely used herbicide diuron to a green alga, Pseudokirchneriella subcapitata

  • Rumana Tasmin
  • Yohei Shimasaki
  • Michito Tsuyama
  • Xuchun Qiu
  • Fatma Khalil
  • Nozomu Okino
  • Naotaka Yamada
  • Shinji Fukuda
  • Ik-Joon Kang
  • Yuji Oshima
Research Article


In the actual environment, temperatures fluctuate drastically through season or global warming and are thought to affects risk of pollutants for aquatic biota; however, there is no report about the effect of water temperature on toxicity of widely used herbicide diuron to fresh water microalgae. The present research investigated inhibitory effect of diuron on growth and photosynthetic activity of a green alga Pseudokirchneriella subcapitata at five different temperatures (10, 15, 20, 25, and 30 °C) for 144 h of exposure. As a result, effective diuron concentrations at which a 50 % decrease in algal growth occurred was increased with increasing water temperature ranging from 9.2 to 20.1 μg L–1 for 72 h and 9.4–28.5 μg L–1 for 144 h. The photochemical efficiency of photosystem II (F v/F m ratio) was significantly reduced at all temperatures by diuron exposure at 32 μg L–1 after 72 h. Inhibition rates was significantly increased with decreased water temperature (P < 0.01). Intracellular H2O2 levels as an indicator of oxidative stress were also decreased with increasing temperature in both control and diuron treatment groups and were about 2.5 times higher in diuron treatment groups than that of controls (P < 0.01). Our results suggest water temperatures may affect the toxicokinetics of diuron in freshwater and should therefore be considered in environmental risk assessment.


Diuron Pseudokirchneriella subcapitata Water temperature Photosynthetic activity Intracellular H2O2 



The cost of publication was supported in part by the Research Grant for Young Investigators of the Faculty of Agriculture, Kyushu University, Japan. This study was also supported in part by the JSPS Core-to-Core Program (B. Asia-Africa Science Platforms) “Collaborative Project for Soil and Water Conservation in Southeast Asian Watersheds”.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Rumana Tasmin
    • 1
  • Yohei Shimasaki
    • 1
  • Michito Tsuyama
    • 2
  • Xuchun Qiu
    • 1
  • Fatma Khalil
    • 1
  • Nozomu Okino
    • 3
  • Naotaka Yamada
    • 4
  • Shinji Fukuda
    • 5
  • Ik-Joon Kang
    • 1
  • Yuji Oshima
    • 1
  1. 1.Laboratory of Marine Environmental Science, Faculty of AgricultureKyushu UniversityFukuokaJapan
  2. 2.Laboratory of Silviculture, Faculty of AgricultureKyushu UniversityFukuokaJapan
  3. 3.Laboratory of Marine Resource Chemistry, Faculty of AgricultureKyushu UniversityFukuokaJapan
  4. 4.Laboratory of Pesticide Chemistry, Faculty of AgricultureKyushu UniversityFukuokaJapan
  5. 5.Laboratory of Water Environment Engineering, Faculty of AgricultureKyushu UniversityFukuokaJapan

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