Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

  • Contaminated Land, Ecological Assessment and Remediation Conference Series (CLEAR 2012) : Environmental Pollution and Risk Assessments
  • Published:

Arbuscular mycorrhizal fungi induced differential Cd and P phytoavailability via intercropping of upland kangkong (Ipomoea aquatica Forsk.) with Alfred stonecrop (Sedum alfredii Hance): post-harvest study

  • 1913 Accesses

  • 2 Citations

Abstract

A post-harvest experiment was conducted further to our previous greenhouse pot study on upland kangkong (Ipomoea aquatica Forsk.) and Alfred stonecrop (Sedum alfredii Hance) intercropping system in Cd-contaminated soil inoculated with arbuscular mycorrhizal (AM) fungi. Previously, four treatments were established in the intercropping experiment, including monoculture of kangkong (control), intercropping with stonecrop (IS), and IS plus inoculation with Glomus caledonium (IS + Gc) or Glomus versiforme (IS + Gv). Both kangkong and stonecrop plants were harvested after growing for 8 weeks. Then, the tested soils were reclaimed for growing post-harvest kangkong for 6 weeks. In the post-harvest experiment, there were no significant differences between the IS and control treatments, except for a significantly decreased (p < 0.05) soil available P concentration with IS treatment. Compared with IS, both IS + Gc and IS + Gv significantly decreased (p < 0.05) soil DTPA-extractable (phytoavailable) Cd concentrations, but not total Cd, by elevating soil pH, causing significantly lower (p < 0.05) Cd concentrations in both the root and shoot of kangkong. In addition, both Gc and Gv significantly increased (p < 0.05) soil acid phosphatase activities and available P concentrations and hence resulted in significantly higher (p < 0.05) plant P acquisitions. However, only Gv significantly increased (p < 0.05) kangkong yield, while Gc only significantly elevated (p < 0.05) the shoot P concentration. It suggested that AM fungi have played key roles in Cd stabilization and P mobilization in the intercropping system, and such positive responses seemed to be sustainable and valuable in post-harvest soils.

This is a preview of subscription content, log in to check access.

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Fig. 1
Fig. 2

References

  1. Allen SE (1974) Chemical analysis of ecological materials. Wiley, New York

  2. AQSIQ (2001) Safety qualification for agricultural product—safety requirements for non-environmental pollution vegetable (GB 18406.1-2001). General Administration of Quality Supervision, Inspection and Quarantine of China, Beijing

  3. Bago B, Vierheilig H, Piché Y, Azcon-Aguilar C (1996) Nitrate depletion and pH changes induced by the extraradical mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown in monoxenic culture. New Phytol 133:273–280

  4. Bai JF, Lin XG, Yin R, Zhang HY, Wang JH, Chen XM, Luo YM (2008) The influence of arbuscular mycorrhizal fungi on As and P uptake by maize (Zea mays L.) from As-contaminated soils. Appl Soil Ecol 38:137–145

  5. Baker DE, Amacher MC (1982) Nickel, copper, zinc, and cadmium. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analyses, part 2, chemical and microbiological properties, 2nd edn. American Society of Agronomy, Madison, pp 323–365

  6. Bray RH, Kurtz LT (1945) Determination of total organic and available forms of phosphorus in soils. Soil Sci 59:39–45

  7. Cobb GP, Sands K, Waters M, Wixson BG, Dorward-King E (2000) Accumulation of heavy metals by vegetables grown in mine wastes. Environ Toxicol Chem 19:600–607

  8. Dai J, Becquer T, Rouiller JH, Reversat G, Bernhard-Reversat F, Nahmani J, Lavelle P (2004) Heavy metal accumulation by two earthworm species and its relationship to total and DTPA-extractable metals in soils. Soil Biol Biochem 36:91–98

  9. Dong Y, Zhu YG, Smith FA, Wang YS, Chen BD (2008) Arbuscular mycorrhiza enhanced arsenic resistance of both white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.) plants in an arsenic-contaminated soil. Environ Pollut 155:174–181

  10. Dickinson N, Baker A, Doronila A, Laidlaw S, Reeves R (2009) Phytoremediation of inorganics: realism and synergies. Int J Phytoremediation 11:97–114

  11. Garbisu C, Alkorta I (2001) Phytoextraction: a cost-effective plant-based technology for the removal of metals from the environment. Bioresour Technol 77:229–236

  12. Giasson P, Karam A, Jaouich A (2008) Arbuscular mycorrhizae and alleviation of soil stresses on plant growth. In: Siddiqui ZA, Akhtar MS, Futai K (eds) Mycorrhizae: sustainable agriculture and forestry. Springer, Dordrecht, pp 99–134

  13. Gove B, Hutchinson JJ, Young SD, McGrath SP (2002) Uptake of metals by plants sharing a rhizosphere with the hyperaccumulator Thlaspi caerulescens. Int J Phytoremediation 4:267–281

  14. Hanson WC (1950) The photometric determination of phosphorus in fertilisers using the phosphovanado–molybdate complex. J Sci Food Agric 1:172–173

  15. Hu J, Chan PT, Wu F, Wu S, Zhang J, Lin X, Wong MH (2013) Arbuscular mycorrhizal fungi induce differential Cd and P acquisition by Alfred stonecrop (Sedum alfredii Hance) and upland kangkong (Ipomoea aquatica Forsk.) in an intercropping system. Appl Soil Ecol 63:29–35

  16. Hu J, Lin X, Wang J, Cui X, Dai J, Chu H, Zhang J (2010) Arbuscular mycorrhizal fungus enhances P-acquisition of wheat (Triticum aestivum L.) in a sandy loam soil with long-term inorganic fertilization regime. Appl Microbiol Biotechnol 88:781–787

  17. Khan AG (2005) Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soils in phytoremediation. J Trace Elem Med Biol 18:355–364

  18. Li H, Ye ZH, Chan WF, Chen XW, Wu FY, Wu SC, Wong MH (2011) Can arbuscular mycorrhizal fungi improve grain yield, As uptake and tolerance of rice grown under aerobic conditions? Environ Pollut 159:2537–2545

  19. Li JT, Qiu JW, Wang XW, Zhong Y, Lan CY, Shu WS (2006) Cadmium contamination in orchard soils and fruit trees and its potential health risk in Guangzhou, China. Environ Pollut 143:159–165

  20. Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London

  21. McGrath SP, Lombi E, Gray CW, Caille N, Dunham SJ, Zhao FJ (2006) Field evaluation of Cd and Zn phytoextraction potential by the hyperaccumulators Thlaspi caerulescens and Arabidopsis halleri. Environ Pollut 141:115–125

  22. Requena N (2005) Measuring quality of service: phosphate ‘à la carte’ by arbuscular mycorrhizal fungi. New Phytol 157:555–567

  23. Rodriguez O, Sellers G, Sinnett D, Moffat A, Hutchings T (2010) Use of remediated soil materials for sustainable plant growth. Land Contam Reclam 18:25–39

  24. Sadowsky MJ (1999) Phytoremediation: past promises and future practices. In: Bell CR, Brylinsky M, Johnson-Green P (eds) Proceedings of the 8th international symposium on microbial ecology. Atlantic Canada Society for Microbial Ecology, Halifax, pp 1–7

  25. Sand S, Becker W (2012) Assessment of dietary cadmium exposure in Sweden and population health concern including scenario analysis. Food Chem Toxicol 50:536–544

  26. SEPAC (1995) Environment quality standard for soils (GB 15618–1995). State Environmental Protection Administration of China, Beijing

  27. Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analyses, part 2, chemical and microbiological properties, 2nd edn. American Society of Agronomy, Madison, pp 903–947

  28. ter Braak CJF, Prentice IC (1988) A theory of gradient analysis. Adv Ecol Res 34:235–282

  29. Van Nevel L, Mertens J, Oorts K, Verheyen K (2007) Phytoextraction of metals from soils: how far from practice? Environ Pollut 150:34–40

  30. Wagner GJ (1993) Accumulation of cadmium in crop plants and its consequences to human health. Adv Agron 51:173–212

  31. Wang FY, Lin XG, Yin R (2007a) Inoculation with arbuscular mycorrhizal fungus Acaulospora mellea decreases Cu phytoextraction by maize from Cu-contaminated soil. Pedobiologia 51:99–109

  32. Wang FY, Lin XG, Yin R (2007b) Role of microbial inoculation and chitosan in phytoextraction of Cu, Zn, Pb and Cd by Elsholtzia splendens—a field case. Environ Pollut 147:248–255

  33. Wang FY, Lin XG, Yin R, Wu LH (2006) Effects of arbuscular mycorrhizal inoculation on the growth of Elsholtzia splendens and Zea mays and the activities of phosphatase and urease in a multi-metal-contaminated soil under unsterilized conditions. Appl Soil Ecol 31:110–119

  34. Yang XE, Long XX, Ye HB, Calvert DV, Stoffella PJ (2004) Cadmium tolerance and hyperaccumulation in a new Zn-hyperaccumulating plant species (Sedum alfredii Hance). Plant Soil 259:181–189

  35. Yang Y, Zhang FS, Li HF, Jiang RF (2009) Accumulation of cadmium in the edible parts of six vegetable species grown in Cd-contaminated soils. J Environ Manage 90:1117–1122

Download references

Acknowledgments

We wish to acknowledge Dr. Bing Li, Mr. Zhiyun Dai, and Mr. Xun Wang of Sun Yat-sen University for their assistance in field sampling and Mr. King Wai Chan, Dr. Ho Man Leung, and Mr. Cheung Lung Lam of Hong Kong Baptist University for their assistance in greenhouse experiment and sample analysis. We are also grateful to Ms. Sue Fung and two anonymous reviewers for their useful comments and suggestions on manuscript revision and English editing. This work was supported by the General Research Fund (HKBU 261510) and Special Equipment Grant (SEG HKBU09) of the Research Grants Council of Hong Kong and the Mini-AoE (Area of Excellence) Fund (RC/AOE/08-09/01) of Hong Kong Baptist University.

Author information

Correspondence to Xiangui Lin or Ming Hung Wong.

Additional information

Responsible editor: Elena Maestri

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hu, J., Li, J., Wu, F. et al. Arbuscular mycorrhizal fungi induced differential Cd and P phytoavailability via intercropping of upland kangkong (Ipomoea aquatica Forsk.) with Alfred stonecrop (Sedum alfredii Hance): post-harvest study. Environ Sci Pollut Res 20, 8457–8463 (2013). https://doi.org/10.1007/s11356-013-1903-7

Download citation

Keywords

  • DTPA-extractable Cd
  • Glomus caledonium
  • Glomus versiforme
  • Metal stabilization
  • Phytoremediation
  • Soil acid phosphatase