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  • Contaminated Land, Ecological Assessment and Remediation Conference Series (CLEAR 2012) : Environmental Pollution and Risk Assessments
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Arbuscular mycorrhizal fungi induced differential Cd and P phytoavailability via intercropping of upland kangkong (Ipomoea aquatica Forsk.) with Alfred stonecrop (Sedum alfredii Hance): post-harvest study

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A post-harvest experiment was conducted further to our previous greenhouse pot study on upland kangkong (Ipomoea aquatica Forsk.) and Alfred stonecrop (Sedum alfredii Hance) intercropping system in Cd-contaminated soil inoculated with arbuscular mycorrhizal (AM) fungi. Previously, four treatments were established in the intercropping experiment, including monoculture of kangkong (control), intercropping with stonecrop (IS), and IS plus inoculation with Glomus caledonium (IS + Gc) or Glomus versiforme (IS + Gv). Both kangkong and stonecrop plants were harvested after growing for 8 weeks. Then, the tested soils were reclaimed for growing post-harvest kangkong for 6 weeks. In the post-harvest experiment, there were no significant differences between the IS and control treatments, except for a significantly decreased (p < 0.05) soil available P concentration with IS treatment. Compared with IS, both IS + Gc and IS + Gv significantly decreased (p < 0.05) soil DTPA-extractable (phytoavailable) Cd concentrations, but not total Cd, by elevating soil pH, causing significantly lower (p < 0.05) Cd concentrations in both the root and shoot of kangkong. In addition, both Gc and Gv significantly increased (p < 0.05) soil acid phosphatase activities and available P concentrations and hence resulted in significantly higher (p < 0.05) plant P acquisitions. However, only Gv significantly increased (p < 0.05) kangkong yield, while Gc only significantly elevated (p < 0.05) the shoot P concentration. It suggested that AM fungi have played key roles in Cd stabilization and P mobilization in the intercropping system, and such positive responses seemed to be sustainable and valuable in post-harvest soils.

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We wish to acknowledge Dr. Bing Li, Mr. Zhiyun Dai, and Mr. Xun Wang of Sun Yat-sen University for their assistance in field sampling and Mr. King Wai Chan, Dr. Ho Man Leung, and Mr. Cheung Lung Lam of Hong Kong Baptist University for their assistance in greenhouse experiment and sample analysis. We are also grateful to Ms. Sue Fung and two anonymous reviewers for their useful comments and suggestions on manuscript revision and English editing. This work was supported by the General Research Fund (HKBU 261510) and Special Equipment Grant (SEG HKBU09) of the Research Grants Council of Hong Kong and the Mini-AoE (Area of Excellence) Fund (RC/AOE/08-09/01) of Hong Kong Baptist University.

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Correspondence to Xiangui Lin or Ming Hung Wong.

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Responsible editor: Elena Maestri

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Hu, J., Li, J., Wu, F. et al. Arbuscular mycorrhizal fungi induced differential Cd and P phytoavailability via intercropping of upland kangkong (Ipomoea aquatica Forsk.) with Alfred stonecrop (Sedum alfredii Hance): post-harvest study. Environ Sci Pollut Res 20, 8457–8463 (2013).

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  • DTPA-extractable Cd
  • Glomus caledonium
  • Glomus versiforme
  • Metal stabilization
  • Phytoremediation
  • Soil acid phosphatase