Fast determination of sulfonamides and their acetylated metabolites from environmental water based on magnetic molecularly imprinted polymers
- 554 Downloads
Group-selective magnetic molecularly imprinted polymers (MMIPs) that can extract four widely used sulfonamide antibiotics and their acetylated metabolites from environmental water were synthesized in this study. The MMIPs with saturation magnetization value of 16.7 emu g-1 could be separated from the environmental water samples easily by the application of an adscititious magnetic field, reducing the time consumption of pretreatment. The extraction conditions were evaluated, and optimal extraction conditions were as follows: extraction time, 25 min; amount of polymers, 90 mg; washing solvent, 30 % methanol aqueous solution; and elution solvent, methanol–acetic acid (95:5, v/v). The target analytes were detected by liquid chromatography–tandem mass spectrometry, and the detection limits of the method are in the range of 0.38–1.32 ng L-1. The relative standard deviations of intra- and inter-day are in the range of 1.3–6.8 % and 1.7–9.1 %, respectively. The proposed method is suitable for the analysis of environmental water samples.
KeywordsSulfonamides Acetylated metabolites Environmental water Molecularly imprinted polymer Magnetic Scatchard analysis Matrix effect Liquid chromatography–tandem mass spectrometry
The authors would like to thank the National Natural Science Fund of China (Grant No. 51078108), the Program for New Century Excellent Talents in University (Grant No. NCET-11-0795), and the Development Program of the Ministry of Science and Technology of Jilin Province (Grant No. 20120327) for their support in this study.
- García-Galán MJ, Garrido T, Fraile J, Ginebreda A, Díaz-Cruz MS, Barceló D (2011) Application of fully automated online solid phase extraction–liquid chromatography-electrospray–tandem mass spectrometry for the determination of sulfonamides and their acetylated metabolites in groundwater. Anal Bioanal Chem 399:795–806CrossRefGoogle Scholar
- Koeber R, Fleischer C, Lanza F, Boos KS, Sellergren B, Barceló D (2001) Evaluation of a multidimensional solid-phase extraction platform for highly selective on-line cleanup and high-throughput LC-MS analysis of triazines in river water samples using molecularly imprinted polymers. Anal Chem 73:2437–2444CrossRefGoogle Scholar
- Pailler JY, Krein A, Pfister L, Hoffmann L, Guignard C (2009) Solid phase extraction coupled to liquid chromatography–tandem mass spectrometry analysis of sulfonamides, tetracyclines, analgesics and hormones in surface water and wastewater in Luxembourg. Sci Total Environ 407:4736–4743CrossRefGoogle Scholar
- Stoob K, Singer HP, Goetz CW, Ruff M, Mueller SR (2005) Fully automated online solid phase extraction coupled directly to liquid chromatography–tandem mass spectrometry: Quantification of sulfonamide antibiotics, neutral and acidic pesticides at low concentrations in surface waters. J Chromatogr A 1097:138–147CrossRefGoogle Scholar