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Environmental Science and Pollution Research

, Volume 20, Issue 10, pp 7046–7056 | Cite as

Dechlorination and organohalide-respiring bacteria dynamics in sediment samples of the Yangtze Three Gorges Reservoir

  • Irene Kranzioch
  • Claudia Stoll
  • Andreas Holbach
  • Hao Chen
  • Lijing Wang
  • Binghui Zheng
  • Stefan Norra
  • Yonghong Bi
  • Karl-Werner Schramm
  • Andreas Tiehm
Processes and Environmental Quality in the Yangtze River System

Abstract

Several groups of bacteria such as Dehalococcoides spp., Dehalobacter spp., Desulfomonile spp., Desulfuromonas spp., or Desulfitobacterium spp. are able to dehalogenate chlorinated pollutants such as chloroethenes, chlorobenzenes, or polychlorinated biphenyls under anaerobic conditions. In order to assess the dechlorination potential in Yangtze sediment samples, the presence and activity of the reductively dechlorinating bacteria were studied in anaerobic batch tests. Eighteen sediment samples were taken in the Three Gorges Reservoir catchment area of the Yangtze River, including the tributaries Jialing River, Daning River, and Xiangxi River. Polymerase chain reaction analysis indicated the presence of dechlorinating bacteria in most samples, with varying dechlorinating microbial community compositions at different sampling locations. Subsequently, anaerobic reductive dechlorination of tetrachloroethene (PCE) was tested after the addition of electron donors. Most cultures dechlorinated PCE completely to ethene via cis-dichloroethene (cis-DCE) or trans-dichloroethene. Dehalogenating activity corresponded to increasing numbers of Dehalobacter spp., Desulfomonile spp., Desulfitobacterium spp., or Dehalococcoides spp. If no bacteria of the genus Dehalococcoides spp. were present in the sediment, reductive dechlorination stopped at cis-DCE. Our results demonstrate the presence of viable dechlorinating bacteria in Yangtze samples, indicating their relevance for pollutant turnover.

Keywords

Yangtze Tetrachloroethene Reductive dechlorination Desulfitobacterium spp. Dehalococcoides spp. qPCR 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support from the German Ministry of Education and Research (BMBF, grant no.: 02WT1130). This study is part of the Sino-German Yangtze-Hydro Project (www.yangtze-project.de).

Supplementary material

11356_2013_1545_MOESM1_ESM.doc (1.6 mb)
ESM 1 (DOC 1616 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Irene Kranzioch
    • 1
  • Claudia Stoll
    • 1
  • Andreas Holbach
    • 2
  • Hao Chen
    • 3
  • Lijing Wang
    • 3
  • Binghui Zheng
    • 3
  • Stefan Norra
    • 4
  • Yonghong Bi
    • 5
    • 6
  • Karl-Werner Schramm
    • 6
    • 7
  • Andreas Tiehm
    • 1
  1. 1.Department Environmental BiotechnologyDVGW-Technologiezentrum Wasser (TZW)KarlsruheGermany
  2. 2.Institute of Mineralogy and GeochemistryKarlsruhe Institute of Technology (KIT)KarlsruheGermany
  3. 3.Institute of Water Environment ResearchChinese Research Academy of Environmental Sciences (CRAES)BeijingPeople’s Republic of China
  4. 4.Institute of Geography and GeoecologyKarlsruhe Institute of TechnologyKarlsruheGermany
  5. 5.Institute of HydrobiologyChinese Academy of SciencesWuhanPeople’s Republic of China
  6. 6.Department BiosciencesTechnische Universität München (TUM)FreisingGermany
  7. 7.Molecular EXposomics (MEX), Department of Environmental SciencesHelmholtz Zentrum München–German Research Center for Environmental HealthNeuherbergGermany

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