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Environmental Science and Pollution Research

, Volume 20, Issue 5, pp 2705–2720 | Cite as

Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography–high resolution mass spectrometry

  • Adeline Jondeau-Cabaton
  • Amélie Soucasse
  • Emilien L. Jamin
  • Nicolas Creusot
  • Marina Grimaldi
  • Isabelle Jouanin
  • Sélim Aït-Aïssa
  • Patrick Balaguer
  • Laurent Debrauwer
  • Daniel ZalkoEmail author
Ecotoxicology and Environmental Toxicology : new concepts, new tools

Abstract

Complex mixtures of contaminants with potential adverse effects on human health and wildlife are found in the environment and in the food chain. These mixtures include numerous anthropogenic compounds of various origins and structures, which may behave as endocrine disruptors. Mixture’s complexity is further enhanced by biotic and abiotic transformations. It is therefore necessary to develop new strategies allowing the identification of the structure of known, as well as unknown, nuclear receptor (NR) ligands present in complex matrices. We explored the possibility to use NR-based affinity columns to characterize the presence of bioactive molecules in environmental complex mixtures. Estrogen receptor α (ERα)-based affinity columns were used to trap and purify estrogenic substances present in surface sediment samples collected in a French river under mixed anthropogenic pressure. We combined biological, biochemical and analytical approaches to characterize the structure of ligands retained on columns and demonstrate the presence of known active molecules such as bisphenol A and octylphenol, but also of unexpected ERα ligands (n-butylparaben, hydroxyl-methyl-benzofuranone). High resolution mass spectrometry results demonstrate that ERα affinity columns can be used for the isolation, purification and identification of known as well as unknown estrogenic contaminants present in complex matrices.

Keywords

Environmental pollutants Fresh water sediment Nuclear receptor Endocrine disruptor 

Notes

Acknowledgements

The research was supported by the «Ministère de l'Environnement, du Développement Durable et de la Mer» (MEDDM), « ECOPI » project, Grant P189. The authors want to thank Dr M. Tremblay-Franco for her help regarding statistical analyses and the reviewers for improving the quality of this manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Adeline Jondeau-Cabaton
    • 1
  • Amélie Soucasse
    • 1
  • Emilien L. Jamin
    • 2
  • Nicolas Creusot
    • 3
    • 4
  • Marina Grimaldi
    • 5
  • Isabelle Jouanin
    • 2
  • Sélim Aït-Aïssa
    • 3
  • Patrick Balaguer
    • 5
  • Laurent Debrauwer
    • 2
  • Daniel Zalko
    • 1
    Email author
  1. 1.INRA, UMR 1331 TOXALIM (Research Center in Food Toxicology), Xenobiotic Metabolism Team (MeX)ToulouseFrance
  2. 2.INRA, UMR 1331 TOXALIM (Research Center in Food Toxicology), MetaToul-AXIOMToulouseFrance
  3. 3.INERISUnité Ecotoxicologie in vitro et in vivoVerneuil-en-HalatteFrance
  4. 4.EPOC/LPTC-UMR 5255 CNRS Université Bordeaux 1TalenceFrance
  5. 5.Plateforme CMT-Equipe Signalisation Hormonale, Environnement et Cancer IRCMInstitut de Recherche en Cancérologie de Montpellier INSERM U896-UM1-CRLC Val d’AurelleMontpellier Cedex 5France

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