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Environmental Science and Pollution Research

, Volume 20, Issue 7, pp 4551–4561 | Cite as

Elucidation of lead-induced oxidative stress in Talinum triangulare roots by analysis of antioxidant responses and DNA damage at cellular level

  • Abhay Kumar
  • M. N. V. PrasadEmail author
  • V. Mohan Murali Achary
  • Brahma B. Panda
Research Article

Abstract

Hydroponic experiments were performed with Talinum triangulare (Jacq.) Willd. focusing the root cellular biochemistry with special emphasis on DNA damage, structural, and elemental analyses in Pb(NO3)2 exposed with 0, 0.25, 0.5, 0.75, 1.0, and 1.25 mM for 7 days. Lead (Pb) increased reactive oxygen species production, lipid peroxidation, protein oxidation, cell death, and DNA damage and decreased the protein content in a dose-dependent manner. Likewise, a dose-dependent induction of antioxidative enzymes superoxide dismutase and catalase by Pb was evident. Ascorbate peroxidase on the other hand responded biphasically to Pb treatments by showing induction at low (0.25 and 0.50) and repression at high (0.75–1.25 mM) concentrations. The estimation of proline content also indicated a similar biphasic trend. Scanning electron microscope and energy-dispersive X-ray spectroscopy analysis showed that 1.25 mM Pb treatment resulted in ultrastructural modifications in roots and stem tissue that was marked by the change in the elemental profile. The findings pointed to the role of oxidative stress in the underlying Pb phytotoxicity and genotoxicity in T. triangulare.

Keywords

Antioxidant response Ceylon spinach Comet assay Pb stress Plant bioassay Tissue ultrastructure 

Notes

Acknowledgements

A.K. gratefully acknowledges the University of Hyderabad Research Scholarship through the University Grant Commission, New Delhi. The authors express their gratitude to Mr. S. Pavan Kumar and Ms. Nalini of the Central Instrument Laboratory, UoH for ESEM and confocal studies, respectively, and M. Laxminarayan for helping with the FESEM/EDS study. M.N.V.P. gratefully acknowledge the award of Pitamber Pant National Environment Fellowship by the Ministry of Environment and Forests, GOI, New Delhi (MoEF Ref. No. 17/3/2010-RE Dt 29-2-2012).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Abhay Kumar
    • 1
  • M. N. V. Prasad
    • 1
    Email author
  • V. Mohan Murali Achary
    • 2
  • Brahma B. Panda
    • 2
  1. 1.Department of Plant SciencesUniversity of HyderabadHyderabadIndia
  2. 2.Molecular Biology and Genomics Laboratory, Department of BotanyBerhampur UniversityBerhampurIndia

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