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Environmental Science and Pollution Research

, Volume 20, Issue 2, pp 749–760 | Cite as

A multibiomarker approach on the Atlantic tomcod (Microgadus tomcod) in the St. Lawrence Estuary

  • Célie DupuyEmail author
  • Catherine M. Couillard
  • Jean Laroche
  • Pierre Nellis
  • Pauline Brousseau
  • Michel Fournier
Ecotoxicology of estuaries in France and Québec, Canada

Abstract

A multibiomarker approach was developed on juvenile Atlantic tomcod (Microgadus tomcod) to evaluate the pertinence of this approach for low-cost screening assessment of the environmental quality of various coastal sites within estuaries. Several biometric indices and biomarkers (ethoxyresorufin-O-deethylase (EROD) activity, metallothionein concentration, and immune responses) were investigated on immature and maturing tomcods (≤31 months) collected in four environmentally contrasted sites in the St. Lawrence Estuary (SLE). Simultaneous examination of various age classes provides the opportunity to detect short-term responses in sensitive young-of-the-year fish (e.g., EROD induction) and longer-time effects associated with chronic exposure and bioaccumulation (e.g., metallothionein induction). Principal component analysis was helpful to discriminate between responses possibly related to contaminant exposure (EROD, metallothionein) and responses that could be affected by upstream–downstream gradient (immune response, biometric indices). Measurement of a battery of biomarkers in young tomcods at several sites along the shore of the SLE is a low-cost screening investigation useful to identify hot spots requiring further investigation with chemical analysis and additional reference sites.

Keywords

Biometric indexes EROD Metallothionein Immunotoxicity Pollution Fish 

Notes

Acknowledgments

This work was conducted in the environmental immunotoxicology laboratory at Institut Armand Frappier (IAF-INRS). Financial support has been provided by the Canada Research Chair in Environmental Immunotoxicology (Dr. Michel Fournier) and Collège Doctoral International de l’Université européenne de Bretagne. The authors also thank S. Gauthier-Clerc, Agence Mamu Innu Kaikusseht, and the staff of Parc national Forillon and of the Ministère des Ressources naturelles et de la Faune du Bas-Saint-Laurent for field sampling. The authors thank Marlene Fortier and Benoît Légaré for their technical assistance. Many thanks to an anonymous referee for his pertinent comments on the draft of this paper. This study was also supported by the INTERREG IV program (DIESE): 50 % of the Ph.D. grant was obtained by the first author, for the development of immune markers in ecotoxicology.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Célie Dupuy
    • 1
    • 2
    Email author
  • Catherine M. Couillard
    • 3
  • Jean Laroche
    • 1
  • Pierre Nellis
    • 3
  • Pauline Brousseau
    • 2
  • Michel Fournier
    • 2
  1. 1.Université Européenne de Bretagne, Université de Bretagne occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l’Environnement Marin LEMAR, Institut Universitaire Européen de la MerPlouzanéFrance
  2. 2.Institut National de la Recherche Scientifique-Institut Armand-Frappier (INRS-IAF)LavalCanada
  3. 3.Pêches et Océans CanadaInstitut Maurice LamontagneMont-JoliCanada

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