Environmental Science and Pollution Research

, Volume 20, Issue 2, pp 1117–1123 | Cite as

Cadmium accumulation, activities of antioxidant enzymes, and malondialdehyde (MDA) content in Pistia stratiotes L.

  • Yong Li
  • Shanshan Zhang
  • Wusheng Jiang
  • Donghua Liu
Research Article


The aquatic plant Pistia stratiotes L. (water lettuce) was studied due to its capability of absorption of contaminants in water and its subsequent use in wetlands constructed for wastewater treatment. The effects of Cd on root growth, accumulation of Cd, antioxidant enzymes, and malondialdehyde (MDA) content in P. stratiotes were investigated. The results indicated that P. stratiotes has considerable ability to accumulate Cd. Cadmium induced higher superoxide dismutase (SOD) and peroxidase (POD) activities than catalase activity, suggesting that SOD and POD provided a better defense mechanism against Cd-induced oxidative damage. The accumulation of Cd promoted MDA production.


Pistia stratiotes L. Cadmium (Cd) Lipid peroxidation Antioxidant enzymes 



This project was supported by the National Natural Science Foundation of China. The authors wish to express their appreciation to the reviewers for this paper.


  1. Baker AJM, Brooks RR (1989) Terrestrial higher plants which hyperaccumulate metallic elements—a review of their distribution, ecology and phytochemistry. Biorecovery 1:81–126Google Scholar
  2. Baker AJM, McGrath SP, Reeves RD, Smith JAC (2000) Metal hyperaccumulator plants: a review of the ecology and physiology of a biological resource for phytoremediation of metal-polluted soils. In: Terry N, Bañuelos GS (eds) Phytoremediation of contaminated soil and water, 85B107. CRC, Boca RatonGoogle Scholar
  3. Chaoui A, Mazhoudi S, Ghorbal MH, Ferjani EEL (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus ulgaris L.). Plant Sci 127:139–147CrossRefGoogle Scholar
  4. Dazy M, Masfaraud JF, Férard JF (2009) Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw. Chemosphere 75:297–302CrossRefGoogle Scholar
  5. Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Feller U (2006) Cadmium stress in barley: growth, leaf pigment and protein composition and detoxification of reactive oxygen species. J Plant Nutr 29:451–468CrossRefGoogle Scholar
  6. Duan XC (2003) The study on the trace elements in the free growing vine of harvested tuber dioscoreas. J Guaugxi Normal Univ 21:122–123Google Scholar
  7. Folgar S, Torres E, Pérez-Rama M, Cid A, Herrero C, Abalde J (2009) Dunaliella salina as marine microalga highly tolerant to but a poor remover of cadmium. J Hazard Mater 165:486–493CrossRefGoogle Scholar
  8. Guo T, Zhang G, Zhou M, Wu F, Chen J (2004) Effect of aluminum and cadmium toxicity on growth and antioxidant enzyme activities of two barley genotypes with different A1 resistance. Plant Soil 258:241–248CrossRefGoogle Scholar
  9. Hasan SA, Fariduddin Q, Ali B, Hayat S, Ahmad A (2009) Cadmium: toxicity and tolerance in plants. J Environ Biol 30:165–174Google Scholar
  10. MacRae EA, Ferguson IB (1985) Changes in catalase activity and hydrogen peroxide concentration in plants in response to low temperature. Physiol Plant 65:51–56CrossRefGoogle Scholar
  11. Miretzky P, Saralegui A, Cirelli AF (2004) Aquatic macrophytes potential for the simultaneous removal of heavy metals (Buenos Aires, Argentina). Chemosphere 57:997–1005CrossRefGoogle Scholar
  12. Mishra VK, Tripathi BD (2008) Concurrent removal and accumulation of heavy metals by the three aquatic macrophytes. Bioresource Technol 99:7091–7097CrossRefGoogle Scholar
  13. Mishra S, Srivastava S, Tripathi RD, Govindarajan R, Kuriakose SV, Prasad MNV (2006) Phytochelatin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L. Plant Physiol Biochem 44:25–37CrossRefGoogle Scholar
  14. Mohan BS, Hosetti BB (2006) Phytotoxicity of cadmium on the physiological dynamics of Salvinia natans L. grown in macrophyte ponds. J Environ Biol 27:701–704Google Scholar
  15. Mufarrege MM, Hadad HR, Maine MA (2010) Response of Pistia stratiotes to heavy metals (Cr, Ni, and Zn) and phosphorous. Arch Environ Contam Toxicol 58:53–61CrossRefGoogle Scholar
  16. Odjegba VJ, Fasidi IO (2007) Changes in antioxidant enzyme activities in Eichhornia crassipes (Pontederiaceae) and Pistia stratiotes (Araceae) under heavy metal stress. Rev Biol Trop 55:815–823Google Scholar
  17. Prasad MNV, Strzałka K (1999) Impact of heavy metals on photosynthesis. In: Prasad MNV, Hagemayer J (eds) Heavy metal stress in plants: from molecules to ecosystems. Springer, Berlin, pp 117–139CrossRefGoogle Scholar
  18. Qureshi MI, Abdin MZ, Qadir S, Iqbal M (2007) Leadinduced oxidative stress and metabolic alterations in Cassia angustifolia Vahl. Biol Plant 51:121–128CrossRefGoogle Scholar
  19. Radotic K, Ducic T, Mutavdzic D (2000) Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium. Environ Exp Bot 44:105–113CrossRefGoogle Scholar
  20. Salt DE, Smith RD, Raskin I (1998) Phytoremediation. Annu Rev Plant Physiol Plant Mol Biol 49:643–668CrossRefGoogle Scholar
  21. Sanità di Toppi L, Vurro E, Rossi L, Marabottini R, Musetti R, Careri M, Maffini M, Mucchino C, Corradini C, Badiani M (2007) Different compensatory mechanisms in two metal-accumulating aquatic macrophytes exposed to acute cadmium stress I outdoor artificial lakes. Chemosphere 68:769–780CrossRefGoogle Scholar
  22. Sinha S, Basant A, Malik A, Singh KP (2009) Multivariate modeling of chromium-induced oxidative stress and biochemical changes in plants of Pistia stratiotes L. Ecotoxicology 8:555–566CrossRefGoogle Scholar
  23. Tewari A, Singh R, Singh NK, Rai UN (2008) Amelioration of municipal sludge by Pistia stratiotes L.: role of antioxidant enzymes in detoxification of metals. Bioresource Technol 99:8715–8721CrossRefGoogle Scholar
  24. Vardanyan LG, Ingole BS (2006) Studies on heavy metal accumulation in aquatic macrophytes from Sevan (Armenia) and Carambolim (India) lake systems. Environ Int 32:208–218CrossRefGoogle Scholar
  25. Wang C, Wang LY, Sun Q (2010) Response of phytochelatins and their relationship with cadmium toxicity in floating macrophyte Pistia stratiotes L. at environmentally relevant concentrations. Water Environ Res 82:147–154CrossRefGoogle Scholar
  26. Yu XZ, Gu JD (2007) Hexavalent chromium induced stress and metabolic responses in hybrid willows. Ecotoxicology 16:299–309CrossRefGoogle Scholar
  27. Zhang HY, Jiang YN, He ZY, Ma M (2005) Cadmium accumulation and oxidative burst in garlic (Allium sativum). J Plant Physiol 162:977–984CrossRefGoogle Scholar
  28. Zhang SS, Zhang HM, Qin R, Jiang WS, Liu DH (2009) Cadmium induction of lipid peroxidation and effects on root tip cells and antioxidant enzyme activities in Vicia faba L. Ecotoxicology 18:814–823CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Yong Li
    • 1
    • 2
  • Shanshan Zhang
    • 1
  • Wusheng Jiang
    • 3
  • Donghua Liu
    • 1
  1. 1.Tianjin Key Laboratory of Cytogenetical and Molecular Regulation, College of Life SciencesTianjin Normal UniversityTianjinPeople’s Republic of China
  2. 2.Tianjin Natural History MuseumTianjinPeople’s Republic of China
  3. 3.LibraryTianjin Normal UniversityTianjinPeople’s Republic of China

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