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Isolation and characterization of a Rhodococcus strain with phenol-degrading ability and its potential use for tannery effluent biotreatment

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Abstract

Introduction

Wastewater derived from leather production may contain phenols, which are highly toxic, and their degradation could be possible through bioremediation technologies.

Materials, methods and results

In the present work, microbial degradation of phenol was studied using a tolerant bacterial strain, named CS1, isolated from tannery sediments. This strain was able to survive in the presence of phenol at concentrations of up to 1,000 mg/L. On the basis of morphological and biochemical properties, 16S rRNA gene sequencing, and phylogenetic analysis, the isolated strain was identified as Rhodococcus sp. Phenol removal was evaluated at a lab-scale in Erlenmeyer flasks and at a bioreactor scale in a stirred tank reactor. Rhodococcus sp. CS1 was able to completely remove phenol in a range of 200 to 1,000 mg/L in mineral medium at 30 ± 2 °C and pH 7 as optimal conditions. In the stirred tank bioreactor, we studied the effect of some parameters, such as agitation (200–600 rpm) and aeration (1–3 vvm), on growth and phenol removal efficiency. Faster phenol biodegradation was obtained in the bioreactor than in Erlenmeyer flasks, and maximum phenol removal was achieved at 400 rpm and 1 vvm in only 12 h. Furthermore, Rhodococcus sp. CS1 strain was able to grow and completely degrade phenols from tannery effluents after 9 h of incubation.

Conclusion

Based on these results, Rhodococcus sp. CS1 could be an appropriate microorganism for bioremediation of tannery effluents or other phenol-containing wastewaters.

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Acknowledgements

P.S.G, M.T., and E.A. are members of the research career from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (Argentina). C.E.P. has a fellowship from CONICET-MinCyT Córdoba. We wish to thank PPI (SECyT-UNRC), CONICET, MinCyT Córdoba, and PICT (FONCyT) for the financial support.

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Authors and Affiliations

  1. Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, CP 5800, Río Cuarto (Córdoba), Argentina

    Cintia E. Paisio, Melina A. Talano, Paola S. González & Elizabeth Agostini

  2. Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, CP 1113, Buenos Aires, Argentina

    Víctor D. Busto & Julián Rodríguez Talou

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  1. Cintia E. Paisio
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  2. Melina A. Talano
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  3. Paola S. González
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  4. Víctor D. Busto
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  5. Julián Rodríguez Talou
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  6. Elizabeth Agostini
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Correspondence to Elizabeth Agostini.

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Communicated by: Philippe Garrigues

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Paisio, C.E., Talano, M.A., González, P.S. et al. Isolation and characterization of a Rhodococcus strain with phenol-degrading ability and its potential use for tannery effluent biotreatment. Environ Sci Pollut Res 19, 3430–3439 (2012). https://doi.org/10.1007/s11356-012-0870-8

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  • DOI: https://doi.org/10.1007/s11356-012-0870-8

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