Environmental Science and Pollution Research

, Volume 19, Issue 5, pp 1719–1727 | Cite as

Genotoxic effect of ciprofloxacin during photolytic decomposition monitored by the in vitro micronucleus test (MNvit) in HepG2 cells

  • Manuel Garcia-Käufer
  • Tarek Haddad
  • Marlies Bergheim
  • Richard Gminski
  • Preeti Gupta
  • Nupur Mathur
  • Klaus Kümmerer
  • Volker Mersch-Sundermann
Research Article



Ciprofloxacin (CIP), a broad-spectrum, second-generation fluoroquinolone, has frequently been found in hospital wastewaters and effluents of sewage treatment plants. CIP is scarcely biodegradable, has toxic effects on microorganisms and is photosensitive. The aim of this study was to assess the genotoxic potential of CIP in human HepG2 liver cells during photolysis.


Photolysis of CIP was performed in aqueous solution by irradiation with an Hg lamp, and transformation products were monitored by HPLC-MS/MS and by the determination of dissolved organic carbon (DOC). The cytotoxicity and genotoxicity of CIP and of the irradiated samples were determined after 24 h of exposure using the WST-1 assay and the in vitro micronucleus (MN) test in HepG2 cells.


The concentration of CIP decreased during photolysis, whereas the content of DOC remained unchanged. CIP and its transformation products were not cytotoxic towards HepG2 cells. A concentration-dependent increase of MN frequencies was observed for the parent compound CIP (lowest observed effect level, 1.2 μmol L−1). Furthermore, CIP and the irradiated samples were found to be genotoxic with a significant increase relative to the parent compound after 32 min (P < 0.05). A significant reduction of genotoxicity was found after 2 h of irradiation (P < 0.05).


Photolytic decomposition of aqueous CIP leads to genotoxic transformation products. This proves that irradiated samples of CIP are able to exert heritable genotoxic effects on human liver cells in vitro. Therefore, photolysis as a technique for wastewater treatment needs to be evaluated in detail in further studies, not only for CIP but in general.


Ciprofloxacin Fluoroquinolones Wastewater treatment Photo-oxidation Photolysis Transformation products Hepatoma cell line (HepG2) Genotoxicity Cytotoxicity Micronucleus test (MNvit) 


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Manuel Garcia-Käufer
    • 1
  • Tarek Haddad
    • 4
  • Marlies Bergheim
    • 1
  • Richard Gminski
    • 1
  • Preeti Gupta
    • 2
  • Nupur Mathur
    • 3
  • Klaus Kümmerer
    • 4
  • Volker Mersch-Sundermann
    • 1
  1. 1.Department of Environmental Health SciencesUniversity Medical Centre FreiburgFreiburgGermany
  2. 2.Environmental Toxicology Unit, Department of ZoologyUniversity of RajasthanJaipurIndia
  3. 3.Centre for Converging TechnologiesUniversity of RajasthanJaipurIndia
  4. 4.Institute of Environmental ChemistryLeuphana University LüneburgLüneburgGermany

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