A passive integrated transponder tag implanted by a new alternative surgical method: effects on the oriental weather loach (Misgurnus anguillicaudatus) and application in a small irrigation system
We validated the effects of a passive integrated transponder (PIT) tagging process on the oriental weather loach Misgurnus anguillicaudatus. Laboratory experiments were conducted to assess the effects of PIT tagging on fish survival, growth, wound healing, and tag omission. Two tagging protocols, standard syringe injection versus insertion through a small hole pierced by a fine needle-shaped awl, were compared using a 12.5 × 2.07 mm2 tag. A control group was also included. In comparison with the awl technique, syringe injection heightened the mortality of the loach and delayed healing of the wound caused by tag insertion. No effects of either PIT tagging method were detected on the growth of surviving loach. We also field-tested similarly tagged populations within a river-based irrigation system of Sado Island, Japan. Two different sized tags (long, 12.5 × 2.07 mm2; short, 8.5 × 2.12 mm2) were compared by using antenna loggers which detected fish movement through gates and automatically logged tagged fish’s tag IDs and timestamps. By comparing logged data and actual fish collection surveys both below and above the gates, 77% and 30% of actual loach movements were confirmed to have been successfully logged for the long and short tags, respectively. The awl insertion technique with the longer tag is therefore recommended for use in similar studies of smaller fish species.
KeywordsAntenna gate Individual recognition Passive integrated transponder (PIT) tag Population monitoring Syringe
We sincerely thank C. Wood for English proof reading. We are also grateful for assistance provided by S. Ikematsu, M. Ishiuchi, M. Kagehira, K. Miyahara, K. Saitoh, M. Sato, W. Tanaka, and Y. Tsujimoto. This work was supported by the Global Environment Research Fund of the Ministry of the Environment, Japan (subject no. F-072), Grant-in-Aid for Young Scientists B of the Ministry of Education, Culture, Sports, Science, and Technology (subject no. 19710027), and the Global COE Program (Ministry of Education, Culture, Sports, Science, and Technology, Japan; Center of excellence for Asian conservation ecology as a basis of human–nature mutualism).
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