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Experimental Mechanics

, Volume 59, Issue 5, pp 713–724 | Cite as

Diffraction-Assisted Light Field Microscopy for Microtomography and Digital Volume Correlation with Improved Spatial Resolution

  • Z. Pan
  • M. Lu
  • S. XiaEmail author
Article
  • 113 Downloads

Abstract

Light field microscopy (LFM) is capable of ultrafast tomographic reconstruction using a light field image acquired in a single snapshot. However, the axial resolution of LFM is inferior to its lateral resolution and is non-uniform in the axial direction. A diffraction-assisted light field microscopy (DLFM) method is proposed in this work to achieve resolution enhancement over the conventional LFM. DLFM makes use of a transmission diffraction grating inserted between the specimen and microscope objective. Light field images of different diffraction orders are formed on the sensor plane and encode both the spatial and angular information of light rays emanating from the specimen. A wave optics model is developed to derive the point spread functions (PSFs) of DLFM, which are then used to deconvolute the light field images for tomographic reconstruction. Validation tests are performed on both experimental and simulated data and the results show that DLFM effectively improves the axial resolution without compromising the lateral resolution. Furthermore, we show that tomographic reconstruction using DLFM can be combined with digital volume correlation (DVC) to achieve three-dimensional, full-field displacement measurement.

Keywords

Light field Tomography Optical microscopy Diffraction grating Three-dimensional measurement Digital volume correlation 

Notes

Acknowledgements

We gratefully acknowledge the support of the Haythornthwaite Foundation.

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Copyright information

© Society for Experimental Mechanics 2019

Authors and Affiliations

  1. 1.Woodruff School of Mechanical EngineeringGeorgia Institute of TechnologyAtlantaUSA

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