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Phase Dependent Mechanosensitivity in Cardiomyocytes

  • B.J. Williams
  • M.T.A. SaifEmail author
Article
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Abstract

Cardiomyocytes are mechanosensitive. In the functioning heart, discrete sets of cardiac oscillators maintain stable relative phase dynamics and mechanical coupling between each other through the elastic tissue. A few questions that remains elusive to date are, how strong is the coupling and how tunable is their dynamics, whether this coupling is phase dependent, and if so, at what phase of cardiac dynamics is the coupling most dominant. In other words, at which phase of its dynamics a cardiac cell is most sensitive to forces and deformations induced by its neighbors. Here we address these questions by culturing rat cardiomyocytes on a stretchable substrate. We apply cyclic stretch on the substrate with a range of frequencies in the vicinity of the intrinsic beating frequency of the cell cluster. We find that the cell cluster can synchronize its dynamics with that of the substrate within 25% of its intrinsic frequency in less than a minute. However, it takes much longer time to return to its intrinsic frequency after removal of substrate stretch. With increasing substrate frequency, the cluster tends to catch up, and beats with a range of frequencies between the intrinsic and the applied with wide variation in relative phases. This allows us to measure phase dependent mechano-sensitivity of the cardiac cluster to the periodic deformation of the substrate that is critical to produce stable relative phase dynamics. We find that cardiac cells are most mechano sensitive when they are at 1/2 of their phase. This phase dependence might be mediated by the ion channels active at this phase of the dynamics. This study identifies a functional output of sub-second scale mechanotransduction with the potential to enhance or reinforce cardiac contractile dynamics.

Keywords

Cardiomyocyte Phase Coupled oscillator Long range interaction Synchrony 

Notes

Acknowledgements

This project was funded by the National Science Foundation (NSF), Science and Technology Center on Emergent Behaviors in Integrated Cellular Systems (EBICS) Grant CBET-0939511

Author Contributions

B.J.W., and M.T.A.S. conceived the design, analyzed the data, and contributed to the manuscript. B.J.W. performed the experiments. M.T.A.S. directed the research.

References

  1. 1.
    Bers DM (2002) Cardiac excitation–contraction coupling. Nature 415:198–205CrossRefGoogle Scholar
  2. 2.
    Sherwood L (2015) Human physiology: from cells to systems. Cengage Learning, BostonGoogle Scholar
  3. 3.
    Ludwig A et al (1999) Two pacemaker channels from human heart with profoundly different activation kinetics. EMBO J 18:2323–2329CrossRefGoogle Scholar
  4. 4.
    Hu H, Sachs F (1997) Stretch-activated ion channels in the heart. J Mol Cell Cardiol 29:1511–1523CrossRefGoogle Scholar
  5. 5.
    Sachs F (2010) Stretch-activated ion channels: what are they? Physiology 25:50–56CrossRefGoogle Scholar
  6. 6.
    Craelius W, Chen V, El-Sherif N (1988) Stretch activated ion channels in ventricular myocytes. Biosci Rep 8:407–414CrossRefGoogle Scholar
  7. 7.
    Tatsukawa Y, Kiyosue T, Arita M (1997) Mechanical stretch increases intracellular calcium concentration in cultured ventricular cells from neonatal rats. Heart Vessel 12:128–135CrossRefGoogle Scholar
  8. 8.
    Ruwhof C et al (2001) Mechanical stress stimulates phospholipase c activity and intracellular calcium ion levels in neonatal rat cardiomyocytes. Cell calcium 29:73–83CrossRefGoogle Scholar
  9. 9.
    Tang X, Bajaj P, Bashir R, Saif TA (2011) How far cardiac cells can see each other mechanically. Soft Matter 7:6151–6158CrossRefGoogle Scholar
  10. 10.
    Nitsan I, Drori S, Lewis YE, Cohen S, Tzlil S (2016) Mechanical communication in cardiac cell synchronized beating. Nature Phys 12:472–477CrossRefGoogle Scholar
  11. 11.
    D’hooge J et al (2000) Regional strain and strain rate measurements by cardiac ultrasound: principles, implementation and limitations. Eur Heart J Cardiovasc Imaging 1:154–170Google Scholar
  12. 12.
    Ahmed W, Kural MH, Saif T (2010) A novel platform for in situ investigation of cells and tissues under mechanical strain. Acta Biomater 6(8):2979–90CrossRefGoogle Scholar
  13. 13.
    Williams BJ, Anand SV, Rajagopalan J, Saif MTA (2014) A self-propelled biohybrid swimmer at low reynolds number. Nat Commun 5:3081Google Scholar
  14. 14.
    Schwachtgen J-L, Houston P, Campbell C, Sukhatme V, Braddock M (1998) Fluid shear stress activation of egr-1 transcription in cultured human endothelial and epithelial cells is mediated via the extracellular signal-related kinase 1/2 mitogen-activated protein kinase pathway. J Clin Investig 101:2540CrossRefGoogle Scholar
  15. 15.
    Goldstein RE, Polin M, Tuval I (2009) Noise and synchronization in pairs of beating eukaryotic flagella. Phys Rev Lett 168103:103Google Scholar
  16. 16.
    Tops LF, Delgado V, Marsan NA, Bax JJ (2016) Myocardial strain to detect subtle left ventricular systolic dysfunction. Eur J Heart Fail 19(3):19Google Scholar

Copyright information

© Society for Experimental Mechanics 2019

Authors and Affiliations

  1. 1.Mechanical Science and EngineeringUniversity of Illinois at Urbana-Champaign (UIUC)UrbanaUSA
  2. 2.Department of BioengineeringUIUCUrbanaUSA

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