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The effects of strength training session with different types of muscle action on white blood cells counting and Th1/Th2 response

  • Lucas Soares Marcucci-Barbosa
  • Francisco de Assis Dias Martins-Junior
  • Lázaro Fernandes Lobo
  • Mariana Gomes de Morais
  • Felipe José Aidar
  • Erica Leandro Marciano Vieira
  • Albená Nunes-SilvaEmail author
Original Article
  • 21 Downloads

Abstract

Aim

This research investigated the effects of a strength training session with two different types of muscle actions, predominantly concentric or eccentric in the physiological variables, including the counting of white blood cells and inflammatory mediators; and consequently, changes in the Th1/Th2 balance.

Methods

Twelve healthy adult men performed a strength training session, using two different protocols: predominantly concentric with 5 s of the concentric phase by 1 s of the eccentric phase, and a predominantly eccentric with 1 s of the concentric phase by 5 s of the eccentric phase. Blood samples were collected, before, immediately after and 2 h after the end of the session to analyze subpopulations of white blood cells, creatine kinase (CK), irisin and the levels of anti- and pro-inflammatory mediators.

Results

Both strength training protocols were able to increase the heart rate, lactate concentration, rate of perceived exertion and the levels of circulating creatine kinase. The predominantly concentric strength training exercises increased the number of total white blood cells, and neutrophils 2 h after the end of the session. The plasmatic levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), irisin, soluble tumor necrosis factor receptor-1 (sTNFR1) and sTNFR2 did not change after the strength training protocols.

Conclusion

Therefore, the present study demonstrates that a strength training session is able to disturb the body homeostasis.

Keywords

Physical exercise Immune system Strength training Cytokines Concentric and eccentric training Leukocytes 

Notes

Acknowledgements

The authors would like to thank the Pilot Laboratory and Clinical Analysis (LAPAC/UFOP) and Inflammation Immunobiology Laboratory (LABIIN/UFOP). They would also like to especially thank Érica Leandro Marciano Vieira and the Interdisciplinary Laboratory of Medical Investigation (LIIM/UFMG) for all the support in the cytokine analysis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The Ethical Committee of the Federal University of Ouro Preto, MG approved this study (Res. 196/96—CAAE 56307716.2.0000.5150).

Informed consent

All individuals received written information and gave written consent about the risks and benefits of the research.

References

  1. 1.
    Haff GG, Triplett NT (2015) Essentials of strength training and conditioning, 4th edn. Human kinetics, ChampaignGoogle Scholar
  2. 2.
    Pedersen BK, Hoffman-Goetz L (2000) Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev 80:1055–1081CrossRefGoogle Scholar
  3. 3.
    McArdle WD, Katch FI, Katch VL (2006) Essentials of exercise physiology. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  4. 4.
    Kraemer WJ, Ratamess NA (2004) Fundamentals of resistance training: progression and exercise prescription. Med Sci Sports Exerc 36:674–688CrossRefGoogle Scholar
  5. 5.
    Garber CE, Blissmer B, Deschenes MR, Franklin BA, Lamonte MJ, Lee IM, Nieman DC, Swain DP, American College of Sports M. American College of Sports Medicine position stand (2011) Quantity and quality of exercise for developing and maintaining cardiorespiratory, musculoskeletal, and neuromotor fitness in apparently healthy adults: guidance for prescribing exercise. Med Sci Sports Exerc 43:1334–1359CrossRefGoogle Scholar
  6. 6.
    Costill D, Coyle E, Fink W, Lesmes G, Witzmann F (1979) Adaptations in skeletal muscle following strength training. J Appl Physiol 46:96–99CrossRefGoogle Scholar
  7. 7.
    Ebbeling CB, Clarkson PM (1989) Exercise-induced muscle damage and adaptation. Sports Med 7:207–234CrossRefGoogle Scholar
  8. 8.
    Gleeson M, Bishop NC, Stensel DJ, Lindley MR, Mastana SS, Nimmo MA (2011) The anti-inflammatory effects of exercise: mechanisms and implications for the prevention and treatment of disease. Nat Rev Immunol 11:607–615CrossRefGoogle Scholar
  9. 9.
    Benatti FB, Pedersen BK (2015) Exercise as an anti-inflammatory therapy for rheumatic diseases-myokine regulation. Nat Rev Rheumatol 11:86–97CrossRefGoogle Scholar
  10. 10.
    Febbraio MA (2017) Exercise metabolism in 2016: health benefits of exercise—more than meets the eye! Nat Rev Endocrinol 13:72–74CrossRefGoogle Scholar
  11. 11.
    Freidenreich DJ, Volek JS (2012) Immune responses to resistance exercise. Exerc Immunol Rev 18:8–41PubMedGoogle Scholar
  12. 12.
    Zhao G, Zhou S, Davie A, Su Q (2012) Effects of moderate and high intensity exercise on T1/T2 balance. Exerc Immunol Rev 18:98–114PubMedGoogle Scholar
  13. 13.
    Fortunato AK, Pontes WM, De Souza DMS, Prazeres JSF, Marcucci-Barbosa LS, Santos JMM, Veira ÉLM, Bearzoti E, Pinto KMDC, Talvani A (2018) Strength training session induces important changes on physiological, immunological, and inflammatory biomarkers. J Immunol Res.  https://doi.org/10.1155/2018/9675216 CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Farthing JP, Chilibeck PD (2003) The effects of eccentric and concentric training at different velocities on muscle hypertrophy. Eur J Appl Physiol 89:578–586CrossRefGoogle Scholar
  15. 15.
    Jackson AS, Pollock ML (1978) Generalized equations for predicting body density of men. Br J Nutr 40:497–504CrossRefGoogle Scholar
  16. 16.
    Brzycki M (1993) Strength testing—predicting a one-rep max from reps-to-fatigue. J Phys Educ Recreat Dance 64(1):88–90CrossRefGoogle Scholar
  17. 17.
    Robertson RJ, Goss FL, Rutkowski J, Lenz B, Dixon C, Timmer J, Frazee K, Dube J, Andreacci J (2003) Concurrent validation of the OMNI perceived exertion scale for resistance exercise. Med Sci Sports Exerc 35:333–341CrossRefGoogle Scholar
  18. 18.
    Nosaka K, Clarkson PM (1995) Muscle damage following repeated bouts of high force eccentric exercise. Med Sci Sports Exerc 27:1263–1269CrossRefGoogle Scholar
  19. 19.
    Baird MF, Graham SM, Baker JS, Bickerstaff GF (2012) Creatine-kinase-and exercise-related muscle damage implications for muscle performance and recovery. J Nutr Metab.  https://doi.org/10.1155/2012/960363 CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Chapman D, Newton M, Sacco P, Nosaka K (2006) Greater muscle damage induced by fast versus slow velocity eccentric exercise. Int J Sports Med 27:591–598CrossRefGoogle Scholar
  21. 21.
    Schulze-Koops H, Kalden JR (2001) The balance of Th1/Th2 cytokines in rheumatoid arthritis. Best Pract Res Clin Rheumatol 15:677–691CrossRefGoogle Scholar
  22. 22.
    de Souza DC, Matos VA, dos Santos VO, Medeiros IF, Marinho CS, Nascimento PR, Dorneles GP, Peres A, Müller CH, Krause M (2018) Effects of high-intensity interval and moderate-intensity continuous exercise on inflammatory, leptin, IgA, and lipid peroxidation responses in obese males. Front Physiol.  https://doi.org/10.3389/fphys.2018.00567 CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Ibfelt T, Petersen EW, Bruunsgaard H, Sandmand M, Pedersen BK (2002) Exercise-induced change in type 1 cytokine-producing CD8+ T cells is related to a decrease in memory T cells. J Appl Physiol 93:645–648CrossRefGoogle Scholar
  24. 24.
    Xiang L, Rehm KE, Marshall GD Jr (2014) Effects of strenuous exercise on Th1/Th2 gene expression from human peripheral blood mononuclear cells of marathon participants. Mol Immunol 60:129–134CrossRefGoogle Scholar
  25. 25.
    Pedersen B, Bruunsgaard H (2003) Possible beneficial role of exercise in modulating low-grade inflammation in the elderly. Scand J Med Sci Sports 13:56–62CrossRefGoogle Scholar
  26. 26.
    Albrecht E, Norheim F, Thiede B, Holen T, Ohashi T, Schering L, Lee S, Brenmoehl J, Thomas S, Drevon CA (2015) Irisin—a myth rather than an exercise-inducible myokine. Sci Rep 5:8889CrossRefGoogle Scholar
  27. 27.
    Lourenco MV, Frozza RL, de Freitas GB, Zhang H, Kincheski GC, Ribeiro FC, Gonçalves RA, Clarke JR, Beckman D, Staniszewski A (2019) Exercise-linked FNDC5/irisin rescues synaptic plasticity and memory defects in Alzheimer’s models. Nat Med 25:165CrossRefGoogle Scholar
  28. 28.
    Lehmann M, Keul J, Huber G, Da Prada M (1981) Plasma catecholamines in trained and untrained volunteers during graduated exercise. Int J Sports Med 2:143–147CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2019

Authors and Affiliations

  • Lucas Soares Marcucci-Barbosa
    • 1
  • Francisco de Assis Dias Martins-Junior
    • 1
  • Lázaro Fernandes Lobo
    • 1
  • Mariana Gomes de Morais
    • 1
  • Felipe José Aidar
    • 3
  • Erica Leandro Marciano Vieira
    • 2
  • Albená Nunes-Silva
    • 1
    Email author
  1. 1.Laboratório de Inflamação e Imunologia do Exercício (LABIIEX)Centro Desportivo da Universidade Federal de Ouro Preto (CEDUFOP)Ouro PretoBrazil
  2. 2.Laboratório Interdisciplinar de Investigação Médica (LIIM), Faculdade de MedicinaBelo HorizonteBrazil
  3. 3.Grupo de Estudo e Pesquisa em Performance, Esporte Paradesporte e Saúde, Departamento de Educação FísicaUFSAracajuBrazil

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