Administration of vitamin D and aerobic training: recovery of lung apoptosis markers in male rats exposed to hydrogen peroxide
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Apoptosis is one of the indications of programmed cell death (PCD) and is known as a physiological event in multicellular organisms. This study was designed to determine the effect of aerobic training alongside vitamin D supplementation on lung cell apoptosis in male rats exposed to hydrogen peroxide (H2O2).
48 male rats were assigned into six groups: H2O2, (H); H2O2 + D3, (HD); H2O2 + training, (HE); H2O2 + D3 + E, (HDE); dimethyl sulfoxide, (DMSO) and control intact. 1 mmol/kg of H2O2 was injected three times per week. The exercising rats performed the program on a rodent’s treadmill for 8 weeks, 5 days a week. The HD and HDE groups received a daily dose of 0.5 µg for 8 weeks. The lung tissue was exposed and stored at − 80. Then, the RT-PCR method was employed to examine the gene expressions of BAX, BCL2, Caspase-3 and Bcl-2/Bax ratios.
Results indicated that training, as well as a combination of training and vitamin D had a significant effect on BAX, BCL2 and Bel2/Bax ratio in case of H2O2 toxicity. The training and vitamin D groups both had no significant effect on Caspase-3 gene expression.
Based on the results of this research, it can be concluded that regular aerobic training alongside consumption of D3 might result in significant alteration of the genes involved in apoptosis caused by H2O2 presence in lung tissues.
KeywordsApoptosis Oxidative stress BCL2 BAX Caspase-3 Aerobic training Vitamin D
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
For this type of study formal consent is not required.
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