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Sport Sciences for Health

, Volume 15, Issue 1, pp 183–190 | Cite as

The beneficial effect of α-tocopherol succinate supplementation on DNA oxidation induced by intensive exercise training

  • Hossein TaheriChadorneshinEmail author
  • Seyed-Hosein Abtahi-Eivary
  • Meysam Alipour-Raz
  • Zainab Nezamdoost
Original Article
  • 40 Downloads

Abstract

Production of reactive oxygen species (ROS) induced by exercise training yields serious oxidative damage to cellular structures. Antioxidant supplements are widely used to reduce the deleterious effects of such endogenous ROS. This study aimed to investigate the effect of two types of intensive exercise training along with α-tocopherol succinate supplementation on serum levels of 8-oxoguanine DNA glycosylase (OGG1), 8-hydroxy-2′-deoxyguanosine (8-OHdG), creatine kinase (CK), and lactate dehydrogenase (LDH). Forty-two male albino Wistar rats were randomly assigned into sedentary control (SC), sedentary vehicle (SV), sedentary supplementation (SS), continuous exercise (CE), continuous exercise + supplementation (CES), intermittent exercise (IE), and intermittent exercise + supplementation (IES), with six rats in each group. Intensive continuous and intermittent running on treadmill, combined with α-tocopherol succinate supplementation (60 mg/kg/day) was carried out for 6 weeks. Data were analyzed using one-way analysis of variance at P < 0.05 level. α-Tocopherol succinate supplementation increased serum total antioxidant capacity (TAC) in SS, CES and IES groups. CK, LDH, and OGG1 levels increased significantly in CE and IE groups; however, α-tocopherol succinate supplementation reduced these factors dramatically in CES and IES groups. In addition, 8-OHdG level was remarkably lower in CES and IES groups. Taken together, α-tocopherol succinate supplementation can modify oxidative damage to genomic structures induced by intensive exercise training.

Keywords

Intermittent exercise training Continuous exercise training α-Tocopherol succinate 8-Oxoguanine DNA glycosylase 8-Hydroxy-2′-deoxyguanosine Creatine kinase Lactate dehydrogenase 

Notes

Acknowledgements

We thank the staff of the biochemistry laboratory at Gonabad University of Medical Sciences for their valuable assistance with us in carrying out the biochemical assays.

Author contributions

HT, SH-AE, and ZN and conceived the study and its design and coordination. All authors were involved in the data collection, data analysis, and drafting of the manuscript. In addition, all authors read and approved the final version of the manuscript, and agreed with the order of presentation of the authors.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All methods performed in the study were in accordance with ethical standards of the national research committee and with 1964 Helsinki Declaration.

Informed consent

There is no informed consent for this study scince the study was not conducted on humans.

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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  • Hossein TaheriChadorneshin
    • 1
    Email author
  • Seyed-Hosein Abtahi-Eivary
    • 2
  • Meysam Alipour-Raz
    • 1
  • Zainab Nezamdoost
    • 3
  1. 1.Department of Sport SciencesUniversity of BojnordBojnordIran
  2. 2.Department of Clinical BiochemistryGonabad University of Medical SciencesGonabadIran
  3. 3.Department of Sport SciencesUniversity of BirjandBirjandIran

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