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Sport Sciences for Health

, Volume 15, Issue 1, pp 133–139 | Cite as

Biochemical changes in oxidative stress markers following endurance training and consumption of purslane seed in rats with hydrogen peroxide-induced toxicity

  • Rahman SooriEmail author
  • Valiollah Shahedi
  • Ali Akbarnejad
  • Siroos Choobineh
Original Article

Abstract

The current study examined fluctuations in oxidative stress markers following endurance training (ET) and consumption of purslane seeds (Ps) in rats after receiving H2O2. Fifty-four adult male Wistar rats were assigned to nine experimental groups: (1) control (intoxicated-no treatment); (2) ET; (3) ET + Ps 50 mg/kg/day; (4) ET + Ps 200 mg/kg/day; (5) ET + Ps 400 mg/kg/day; (6) Ps 50 mg/kg/day; (7) Ps 200 mg/kg/day; (8) Ps 400 mg/kg/day; (9) control (non-intoxicated, intact). The first eight groups were given 100 mg/kg of H2O2 to induce oxidative stress. Groups 2–5 were given ET for a period of 8 weeks. Heart and lung tissues were then exposed to evaluate the oxidative stress markers. Catalase, glutathione peroxidase, malondialdehyde, and superoxide dismutase enzymes were measured using ELISA kits. A marked improvement in enzyme concentration was observed in both tissues. It was more pronounced in the groups receiving higher doses of Ps + ET. The findings provide evidence that purslane seed supplementation has antioxidant potential alongside endurance training and improved the ability to cope with oxidative stress.

Keywords

Oxidative stress Purslane seed Endurance training Hydrogen peroxide 

Notes

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

None of the authors have financial or other conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed consent

For this type of study, formal consent is not required.

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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  • Rahman Soori
    • 1
    Email author
  • Valiollah Shahedi
    • 1
  • Ali Akbarnejad
    • 1
  • Siroos Choobineh
    • 1
  1. 1.Department of Exercise Physiology, Faculty of Physical Education and Sport SciencesUniversity of TehranTehranIran

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