Biochemical changes in oxidative stress markers following endurance training and consumption of purslane seed in rats with hydrogen peroxide-induced toxicity
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The current study examined fluctuations in oxidative stress markers following endurance training (ET) and consumption of purslane seeds (Ps) in rats after receiving H2O2. Fifty-four adult male Wistar rats were assigned to nine experimental groups: (1) control (intoxicated-no treatment); (2) ET; (3) ET + Ps 50 mg/kg/day; (4) ET + Ps 200 mg/kg/day; (5) ET + Ps 400 mg/kg/day; (6) Ps 50 mg/kg/day; (7) Ps 200 mg/kg/day; (8) Ps 400 mg/kg/day; (9) control (non-intoxicated, intact). The first eight groups were given 100 mg/kg of H2O2 to induce oxidative stress. Groups 2–5 were given ET for a period of 8 weeks. Heart and lung tissues were then exposed to evaluate the oxidative stress markers. Catalase, glutathione peroxidase, malondialdehyde, and superoxide dismutase enzymes were measured using ELISA kits. A marked improvement in enzyme concentration was observed in both tissues. It was more pronounced in the groups receiving higher doses of Ps + ET. The findings provide evidence that purslane seed supplementation has antioxidant potential alongside endurance training and improved the ability to cope with oxidative stress.
KeywordsOxidative stress Purslane seed Endurance training Hydrogen peroxide
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Compliance with ethical standards
Conflict of interest
None of the authors have financial or other conflicts of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
For this type of study, formal consent is not required.
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