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Sport Sciences for Health

, Volume 15, Issue 1, pp 59–63 | Cite as

Long-term effects of sprint interval training on expression of cardiac genes involved in energy efficiency

  • Hossein TaheriChadorneshin
  • Fatemeh Rostamkhani
  • Hossein ShirvaniEmail author
Original Article
  • 26 Downloads

Abstract

Purpose

The real mechanisms of intensive exercise training-induced energy efficiency have not yet been well examined. Therefore, the aim of the present study was to investigate the effects of sprint interval training (SIT) on gene expression of uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS).

Methods

For this purpose, 16 Albino Wistar rats (250–300 g) were randomly divided into equal groups of control and sprint training. The animals run on treadmill for 10 weeks, 5 days per week at intensity corresponding to 90–95% maximal oxygen consumption. The gene expression of UCP2, UCP3 and eNOS was analyzed by RT-PCR method in hearts. The data were analyzed by independent samples T test at P < 0.05 level.

Results

Sprint interval training significantly decreased mRNA expression of UCP2 (t14 = 4.818, P = 0.001) and UCP3 (t14 = 4.620, P = 0.001) in cardiac muscle of rats. In contrast, mRNA expression of eNOS in cardiac muscle significantly increased following sprint interval training (t14 = 7.967, P = 0.001).

Conclusion

This study elucidates that SIT through reduction in gene expression of uncoupling proteins can improve energy efficiency. But, more studies are needed to confirm this hypothesis.

Keyword

Sprint interval training (SIT) Uncoupling proteins Endothelial nitric oxide synthase Energy efficiency 

Abbreviations

C

Control

eNOS

Endothelial nitric oxide synthase

SIT

Sprint interval training

UCPs

Uncoupling proteins

VO2max

Maximal oxygen uptake

Notes

Acknowledgements

We thank the staff of the animal laboratory at Baqiyatallah University of Medical Sciences for their valuable assistance with us in carrying out the exercise protocols and animal surgery.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All methods performed in the study were in accordance with ethical standards of the national research committee and with the 1964 Helsinki Declaration.

Informed consent

There is no informed consent for this study since the study was not conducted on humans.

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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Sport SciencesUniversity of BojnordBojnordIran
  2. 2.Department of Biology, College of Basic Sciences, Yadegar-e-Imam Khomeini (RAH) Shahre Rey BranchIslamic Azad UniversityTehranIran
  3. 3.Exercise Physiology Research Center, Life Style InstituteBaqiyatallah University of Medical SciencesTehranIran

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