Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects
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To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency.
Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (V max), and an affinity constant (K m ) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated.
Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R 2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, V max, and K m values (p = 0.08, 0.12, 0.71, and 0.68, respectively).
Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.
KeywordsSleep bruxism Human serotonin transporter Uptake ability Electromyography (EMG) Peripheral platelet
The Ministry of Education, Science and Culture, Japan, provided financial support in the form of Grant-in-Aid ((B) No. 23390442) and Grant-in-Aid for Exploratory Research (No. 25670819) . The sponsor had no role in the design or conduct of this research.
Conflict of interest
All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements) or non-financial interest (such as personal or professional relationships, affiliations, knowledge, or beliefs) in the subject matter or materials discussed in this manuscript.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
- 20.Mainieri VC, Saueressig AC, Pattussi MP, Fagondes SC, Grossi ML (2012) Validation of the Bitestrip versus polysomnography in the diagnosis of patients with a clinical history of sleep bruxism. Oral Surg Oral Med Oral Pathol Oral Radiol 113:612–617. doi: 10.1016/j.oooo.2011.10.008 CrossRefPubMedGoogle Scholar
- 28.Abe Y, Suganuma T, Ishii M, Yamamoto G, Gunji T, Clark GT, Tachikawa T, Kiuchi Y, Igarashi Y, Baba K (2012) Association of genetic, psychological and behavioral factors with sleep bruxism in a Japanese population. J Sleep Res 21:289–296. doi: 10.1111/j.1365-2869.2011.00961 CrossRefPubMedGoogle Scholar