Fluorescent Polymer Dots for Tracking SKOV3 Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy
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Probe-based confocal laser endomicroscopy (pCLE) is a novel technique allowing real-time and high-resolution imaging in vivo. It provides microscopic images and increases the penetration depth of tissues compared with conventional white light endoscopy. The aim of the present study was to track ovarian cancer cells in organs by fluorescent polymer dots based on pCLE.
SKOV3-mCherry cells were incubated with polymer dots for 24 h in a serum-free culture medium. Labeled cells were administrated to nude mice via intravenous, intraperitoneal, and lymph node injection. The fluorescent signals of labeled cells in organs were observed by pCLE. Furthermore, the results were confirmed by frozen section analysis.
pCLE displayed fluorescence signals of labeled cells in the vessels of organs. Besides, the accumulations of labeled cells visualized in detoxification organs like the spleen and kidney were increased with time.
In this article, we present a real-time and convenient method for tracking SKOV3-mCherry in living mice by combined fluorescent polymer dots with pCLE.
Key wordsPolymer dots Fluorescence imaging pCLE Tracking Ovarian cancer
This study was supported by grants from the National Key R&D Program of China (2016YFC1303100), the National Natural Science Foundation of China (81671738, 81301261, and 21374059), the Shanghai Pujiang Project (13PJ1405000), and the Medicine-Engineering Cross Project of Shanghai Jiao Tong University (YG2016MS73).
Compliance with Ethical Standards
All animal experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Shanghai Jiao Tong University (Shanghai, China).
Conflict of Interest
The authors declare that they have no conflict of interest.
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