Fluorescent Polymer Dots for Tracking SKOV3 Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy

  • Yufan Zhang
  • Yao Li
  • Yixiao Guo
  • Yidian Yang
  • Shiyi Tang
  • Liqin XiongEmail author
Brief Article



Probe-based confocal laser endomicroscopy (pCLE) is a novel technique allowing real-time and high-resolution imaging in vivo. It provides microscopic images and increases the penetration depth of tissues compared with conventional white light endoscopy. The aim of the present study was to track ovarian cancer cells in organs by fluorescent polymer dots based on pCLE.


SKOV3-mCherry cells were incubated with polymer dots for 24 h in a serum-free culture medium. Labeled cells were administrated to nude mice via intravenous, intraperitoneal, and lymph node injection. The fluorescent signals of labeled cells in organs were observed by pCLE. Furthermore, the results were confirmed by frozen section analysis.


pCLE displayed fluorescence signals of labeled cells in the vessels of organs. Besides, the accumulations of labeled cells visualized in detoxification organs like the spleen and kidney were increased with time.


In this article, we present a real-time and convenient method for tracking SKOV3-mCherry in living mice by combined fluorescent polymer dots with pCLE.

Key words

Polymer dots Fluorescence imaging pCLE Tracking Ovarian cancer 



This study was supported by grants from the National Key R&D Program of China (2016YFC1303100), the National Natural Science Foundation of China (81671738, 81301261, and 21374059), the Shanghai Pujiang Project (13PJ1405000), and the Medicine-Engineering Cross Project of Shanghai Jiao Tong University (YG2016MS73).

Compliance with Ethical Standards

Ethical Approval

All animal experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Shanghai Jiao Tong University (Shanghai, China).

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© World Molecular Imaging Society 2019

Authors and Affiliations

  1. 1.Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  2. 2.The Key Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and SensorsShanghai Normal UniversityShanghaiPeople’s Republic of China

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