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Near-Infrared Spatially Resolved Spectroscopy as an Indirect Technique to Assess Brown Adipose Tissue in Young Women

  • Francisco M. Acosta
  • Jörn Berchem
  • Borja Martinez-Tellez
  • Guillermo Sanchez-Delgado
  • Juan M. A. Alcantara
  • Lourdes Ortiz-Alvarez
  • Takafumi Hamaoka
  • Jonatan R. Ruiz
Research Article
  • 85 Downloads

Abstract

Purpose

Near-infrared spectroscopy (NIRS) has recently been proposed as an indirect technique to assess brown adipose tissue (BAT) in young men. NIRS arises as a novel technique to avoid the limitations of the “gold-standard” 2-deoxy-2-[18F]fluoro-D-glucose ([18F]DG) positron emission tomography combined with X-ray computed tomography (PET/CT). The aim of this study was to examine the association between near-infrared spatially resolved spectroscopy (NIRSRS) parameters and BAT volume and activity estimated by [18F]DG-PET/CT in 18 young healthy women.

Procedures

NIRSRS parameters [tissue saturation index and concentrations of total haemoglobin, oxy-haemoglobin, and deoxy-haemoglobin] were continuously measured in the supraclavicular and forearm regions, in both warm and cold (2 h of personalised cold exposure) conditions. Then, the NIRSRS data were analysed as an average of 5 min in 4 different periods: (i) warm period as the baseline record, (ii) cold period I, (iii) cold period II, and (iv) cold period III. The data were then correlated with BAT volume and activity (SUVmean and SUVpeak) estimated by [18F]DG-PET/CT.

Results

There was no association between the NIRSRS parameters in the supraclavicular region in warm conditions (no previous cold exposure) and BAT volume and activity (P > 0.05). Similarly, the cold-induced changes of the NIRSRS parameters in the supraclavicular region were not associated with BAT volume and activity (P > 0.05).

Conclusions

NIRSRS does not seem to be a valid technique to indirectly assess BAT in young healthy women. Further research is needed to validate this technique against other methods such as PET/CT using different radiotracers or magnetic resonance imaging.

Key words

Acute cold exposure BAT perfusion Cold-induced thermogenesis Energy balance Molecular imaging Oxygen consumption Oxygen delivery 

Notes

Acknowledgments

We are grateful to Ms. Carmen Sainz-Quinn for the assistance with the English language and to Marco Dat (Artinis Medical Systems) for his excellent technical assistance.

Financial Support

The study was supported by the Spanish Ministry of Economy and Competitiveness (PTA 12264-I), Fondo de Investigación Sanitaria del Instituto de Salud Carlos III (PI13/01393), and Retos de la Sociedad (DEP2016-79512-R), Fondos Estructurales de la Unión Europea (FEDER), by the Spanish Ministry of Education (FPU 13/04365 and 15/04059), by the Fundación Iberoamericana de Nutrición (FINUT), by the Redes temáticas de investigación cooperativa RETIC (Red SAMID RD16/0022), by AstraZeneca HealthCare Foundation, and by the University of Granada, Plan Propio de Investigación 2016, Excellence actions: Units of Excellence; Unit of Excellence on Exercise and Health (UCEES). This study is part of a PhD thesis conducted in the Biomedicine Doctoral Studies, University of Granada, Spain.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

11307_2018_1244_MOESM1_ESM.pdf (184 kb)
ESM 1 (PDF 183 kb)

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Copyright information

© World Molecular Imaging Society 2018

Authors and Affiliations

  • Francisco M. Acosta
    • 1
  • Jörn Berchem
    • 1
    • 2
  • Borja Martinez-Tellez
    • 1
    • 3
  • Guillermo Sanchez-Delgado
    • 1
  • Juan M. A. Alcantara
    • 1
  • Lourdes Ortiz-Alvarez
    • 1
  • Takafumi Hamaoka
    • 4
  • Jonatan R. Ruiz
    • 1
  1. 1.PROFITH “PROmoting FITness and Health through physical activity” Research Group, Department of Physical and Sports Education, Faculty of Sports ScienceUniversity of GranadaGranadaSpain
  2. 2.Department of Sports Medicine, Institute of Sports SciencesJustus-Liebig-University GiessenGiessenGermany
  3. 3.Department of Medicine, Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular MedicineLeiden University Medical CenterLeidenThe Netherlands
  4. 4.Department of Sports Medicine for Health PromotionTokyo Medical UniversityTokyoJapan

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