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Molecular Imaging and Biology

, Volume 20, Issue 1, pp 21–26 | Cite as

Influence of Antibiotic Pressure on Five Plasmid-based Bioluminescent Gram-negative Bacterial Strains

  • Xiwen Wang
  • Hang Chi
  • Qianxue Li
  • Wenliang Li
  • Jiakuan Li
  • Bo Li
  • Weicun Gao
  • Da Zhang
  • Yu Sun
  • Le Yi
  • Han Qu
  • Yutian Wang
  • Zhiping LiEmail author
  • Zhiping XiaEmail author
Brief Article

Abstract

Purpose

The present study aims to develop five Gram-negative bacteria expressing bacterial luciferase for use to evaluate the influence of different antibiotics on bacterial bioluminescence.

Procedures

The pBBR-lux plasmid was introduced into five Gram-negative bacteria; the bioluminescent signals and colony-forming unit (CFU)/ml of all the bioluminescent strains were monitored with six antibiotics at various concentrations.

Results

Dose-dependent bioluminescence signals can be used for rapid bacterial antibiotic susceptibility test (AST). All five bioluminescent bacterial strains have similar bioluminescence and CFU enhancement at sub-minimum inhibitory concentration (MIC) of six different antibiotics.

Conclusion

The bioluminescent signals and CFU enhancement at sub-MIC antibiotic concentrations should be of value in the research of new antibiotic drugs and bioluminescent imaging.

Key words

lux CDABE Antibiotics Bioluminescent bacteria 

Notes

Acknowledgments

Financial support for this study came from the National Key Research and Development Program of China (contract no. 2016YFC1201600).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11307_2017_1110_MOESM1_ESM.pdf (576 kb)
ESM 1 (PDF 575 kb)

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Copyright information

© World Molecular Imaging Society 2017

Authors and Affiliations

  • Xiwen Wang
    • 1
  • Hang Chi
    • 1
  • Qianxue Li
    • 1
  • Wenliang Li
    • 2
    • 3
  • Jiakuan Li
    • 1
  • Bo Li
    • 1
  • Weicun Gao
    • 1
  • Da Zhang
    • 1
  • Yu Sun
    • 1
  • Le Yi
    • 1
  • Han Qu
    • 1
  • Yutian Wang
    • 1
  • Zhiping Li
    • 1
    Email author
  • Zhiping Xia
    • 1
    Email author
  1. 1.Key Laboratory of Jinlin Province for Zoonosis Prevention and ControlMilitary Veterinary Institute, AMMSChangchunChina
  2. 2.Jilin Medical UniversityJilinChina
  3. 3.Key Laboratory of Preparation and Application of Environmental Friendly Materials, Ministry of EducationJilin Normal UniversityChangchunChina

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