Quantitative [18F]FMISO PET Imaging Shows Reduction of Hypoxia Following Trastuzumab in a Murine Model of HER2+ Breast Cancer
- First Online:
- Cite this article as:
- Sorace, A.G., Syed, A.K., Barnes, S.L. et al. Mol Imaging Biol (2017) 19: 130. doi:10.1007/s11307-016-0994-1
- 310 Downloads
Evaluation of [18F]fluoromisonidazole ([18F]FMISO)-positron emission tomography (PET) imaging as a metric for evaluating early response to trastuzumab therapy with histological validation in a murine model of HER2+ breast cancer.
Mice with BT474, HER2+ tumors, were imaged with [18F]FMISO-PET during trastuzumab therapy. Pimonidazole staining was used to confirm hypoxia from imaging.
[18F]FMISO-PET indicated significant decreases in hypoxia beginning on day 3 (P < 0.01) prior to changes in tumor size. These results were confirmed with pimonidazole staining on day 7 (P < 0.01); additionally, there was a significant positive linear correlation between histology and PET imaging (r2 = 0.85).
[18F]FMISO-PET is a clinically relevant modality which provides the opportunity to (1) predict response to HER2+ therapy before changes in tumor size and (2) identify decreases in hypoxia which has the potential to guide subsequent therapy.
Key wordsFMISO Oxygenation Vascular maturation Herceptin BT474 Pimonidazole Misonidazole
|Funder Name||Grant Number||Funding Note|
|National Cancer Institute|
|Cancer Prevention and Research Institute of Texas (US)|