Molecular Imaging and Biology

, Volume 18, Issue 5, pp 686–696

Comparative Study of Tumor Targeting and Biodistribution of pH (Low) Insertion Peptides (pHLIP® Peptides) Conjugated with Different Fluorescent Dyes

  • Ramona-Cosmina Adochite
  • Anna Moshnikova
  • Jovana Golijanin
  • Oleg A. Andreev
  • Natallia V. Katenka
  • Yana K. Reshetnyak
Research Article

DOI: 10.1007/s11307-016-0949-6

Cite this article as:
Adochite, RC., Moshnikova, A., Golijanin, J. et al. Mol Imaging Biol (2016) 18: 686. doi:10.1007/s11307-016-0949-6

Abstract

Purpose

Acidification of extracellular space promotes tumor development, progression, and invasiveness. pH (low) insertion peptides (pHLIP® peptides) belong to the class of pH-sensitive membrane peptides, which target acidic tumors and deliver imaging and/or therapeutic agents to cancer cells within tumors.

Procedures

Ex vivo fluorescent imaging of tissue and organs collected at various time points after administration of different pHLIP® variants conjugated with fluorescent dyes of various polarity was performed. Methods of multivariate statistical analyses were employed to establish classification between fluorescently labeled pHLIP® variants in multidimensional space of spectral parameters.

Results

The fluorescently labeled pHLIP® variants were classified based on their biodistribution profile and ability of targeting of primary tumors. Also, submillimeter-sized metastatic lesions in lungs were identified by ex vivo imaging after intravenous administration of fluorescent pHLIP® peptide.

Conclusions

Different cargo molecules conjugated with pHLIP® peptides can alter biodistribution and tumor targeting. The obtained knowledge is essential for the design of novel pHLIP®-based diagnostic and therapeutic agents targeting primary tumors and metastatic lesions.

Key words

Imaging Tumor acidity Fluorescent-guided surgery Targeting of submillimeter metastatic lesions 

Supplementary material

11307_2016_949_MOESM1_ESM.pdf (1.2 mb)
ESM 1(PDF 1278 kb)

Funding information

Funder NameGrant NumberFunding Note
National Institute of General Medical Sciences
  • GM073857
  • GM073857

Copyright information

© World Molecular Imaging Society 2016

Authors and Affiliations

  • Ramona-Cosmina Adochite
    • 1
  • Anna Moshnikova
    • 1
  • Jovana Golijanin
    • 1
  • Oleg A. Andreev
    • 1
  • Natallia V. Katenka
    • 2
  • Yana K. Reshetnyak
    • 1
  1. 1.Physics DepartmentUniversity of Rhode IslandKingstonUSA
  2. 2.Department of Computer Sciences and Statistics, University of Rhode IslandKingstonUSA

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