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Evaluation of Quantitative Imaging Biomarkers in the DSS Colitis Model

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Abstract

Purpose

In humans, colonoscopy is the gold standard for the diagnosis of inflammatory changes of the colon wall. Aim of this study was the identification of less invasive imaging biomarkers in the dextran sodium sulfate (DSS) colitis model to provide additional information on transmural changes of the colon wall.

Procedures

Colitis was induced in C57BL/6 mice by administration of 2, 3, and 4 % DSS over a period of 5 days. Colon wall thickness was measured using magnetic resonance imaging (MRI), ultrasound (US), and x-ray computed tomography (CT), gut inflammation by positron emission tomography/CT, and mucosal changes of the colon wall by colonoscopy. Colon samples were examined histologically.

Results

MRI, CT, US, and histological data revealed increased colon wall thickness in DSS-treated mice compared to healthy controls. Elevated 2-deoxy-2[18F]fluoro-d-glucose uptake and colonoscopy confirmed high inflammatory load in the guts of colitis mice.

Conclusions

The established quantitative imaging readouts offer promising perspectives to develop new compounds and to translate these methods into the clinical setting.

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Abbreviations

CT:

Computed tomography

MRI:

Magnetic resonance imaging

PET/CT:

Positron emission tomography combined with computed tomography

US:

Ultrasound imaging

SPECT:

Single photon emission computed tomography

[18F]FDG:

2-deoxy-2-[18F]fluoro-d-glucose

DSS:

Dextran sodium sulfate

IBD:

Inflammatory bowel disease

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Acknowledgments

We thank Andrea Vögtle, David Kind, and Michael Neumaier for excellent technical assistance and Anna-Lena Martin for statistical analyses.

Author information

Correspondence to Detlef Stiller.

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Conflict of Interest

All the authors are employees of Boehringer Ingelheim Pharma GmbH & Co. K and declare that they have no conflict of interest.

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Beltzer, A., Kaulisch, T., Bluhmki, T. et al. Evaluation of Quantitative Imaging Biomarkers in the DSS Colitis Model. Mol Imaging Biol 18, 697–704 (2016). https://doi.org/10.1007/s11307-016-0937-x

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Key words

  • DSS
  • Mouse model
  • Colitis
  • Inflammatory bowel disease
  • Multimodal imaging