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Molecular Imaging and Biology

, Volume 17, Issue 6, pp 811–818 | Cite as

Establishment of an mKate2-Expressing Cell Line for Non-Invasive Real-Time Breast Cancer In Vivo Imaging

  • Ivan Vuletic
  • Jinghao Liu
  • Honglian Wu
  • Yichen Ding
  • Yu Lei
  • Changhui Li
  • Desheng Zhu
  • Qiushi Ren
  • Hongfang SunEmail author
  • Jun LiEmail author
Research Article

Abstract

Purpose

Non-invasive real-time in vivo imaging experiments using mice as animal models have become crucial for understanding cancer development and treatment. In this study, we have developed and validated a new breast cancer cell line MDA-MB-435s that stably express a far-red fluorescence protein (mKate2) and that could serve as a highly valuable cell model for studying breast cancer detection and therapy using in vivo fluorescence imaging in nude mice.

Procedures

The new cell line (MDA-MB-435s-mKate2) was constructed by plasmid transfection. The stability and sensitivity of mKate2, and the cell biological activities, were tested in vitro using different experimental approaches. For its potential use in tumor growth research and drug therapy in vivo, MDA-MB-435s-mKate2 was validated using the immunocompromised Balb/c nude mice tumor model. In addition, the new cell line has been characterized as a luteinizing hormone-releasing hormone receptor (LHRHR) positive cell line.

Results

Firstly, MDA-MB-435s-mKate2 has shown a stable chromosomal integration of the amplified mKate2 gene and good fluorescence sensitivity for detection using a fluorescence reflectance imaging (FRI) device. Compared to its parental cell line, no significant difference in cell migration, proliferation, and clone formation was observed in vitro. Secondly, using the quantification of tumor-fluorescence surface area in live animals, we were able to monitor and detect the tumor progress or tumor inhibition rate (by Paclitaxel treatment) non-invasively and in real-time. Furthermore, MDA-MB-435s-mKate2 has been positively tested for LHRHR; these findings open the possibility to use this cell line for future studies of breast cancer therapy based on LHRH analogs in vivo.

Conclusion

In the present research, we have successfully built the MDA-MB-435s-mKate2 cell line that can be used as a suitable cell model for breast cancer therapy and anti-cancer drug evaluation by non-invasive fluorescence imaging in mice.

Key words

mKate2 Fluorescence Imaging 

Abbreviations

FRI

Fluorescence reflectance imaging

LHRHR

Luteinizing hormone-releasing hormone receptor

LHRH

Luteinizing hormone-releasing hormone

Notes

Acknowledgements

We acknowledge the financial support by the National Key Instrumentation Development Project (2011YQ030114) and the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (81421004). We would like also to thank Mr. Dong Cao and Mr. DaGang Li for their technical assistance.

Conflict of Interests

The authors declare that they have no conflict of interests.

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Copyright information

© World Molecular Imaging Society 2015

Authors and Affiliations

  • Ivan Vuletic
    • 1
    • 2
  • Jinghao Liu
    • 1
  • Honglian Wu
    • 1
  • Yichen Ding
    • 2
  • Yu Lei
    • 1
  • Changhui Li
    • 2
  • Desheng Zhu
    • 1
  • Qiushi Ren
    • 2
  • Hongfang Sun
    • 2
    Email author
  • Jun Li
    • 1
    Email author
  1. 1.Laboratory Animal CenterPeking UniversityBeijingChina
  2. 2.Department of Biomedical Engineering, College of EngineeringPeking UniversityBeijingChina

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