IND-Directed Safety and Biodistribution Study of Intravenously Injected Cetuximab-IRDye800 in Cynomolgus Macaques
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The use of receptor-targeted antibodies conjugated to fluorophores is actively being explored for real-time imaging of disease states; however, the toxicity of the bioconjugate has not been assessed in non-human primates.
To this end, the in vivo toxicity and pharmacokinetics of IRDye800 conjugated to cetuximab (cetuximab-IRDye800; 21 mg/kg; equivalent to 250 mg/m2 human dose) were assessed in male cynomolgus monkeys over 15 days following intravenous injection and compared with an unlabeled cetuximab-dosed control group.
Cetuximab-IRDye800 was well tolerated. There were no infusion reactions, adverse clinical signs, mortality, weight loss, or clinical histopathology findings. The plasma half-life for the cetuximab-IRDye800 and cetuximab groups was equivalent (2.5 days). The total recovered cetuximab-IRDye800 in all tissues at study termination was estimated to be 12 % of the total dose. Both cetuximab-IRDye800 and cetuximab groups showed increased QTc after dosing. The QTc for the cetuximab-dosed group returned to baseline by day 15, while the QTc of the cetuximab-IRDye800 remained elevated compared to baseline.
IRDye800 in low molar ratios does not significantly impact cetuximab half-life or result in organ toxicity. These studies support careful cardiac monitoring (ECG) for human studies using fluorescent dyes.
Key wordsCetuximab IRDye800 Toxicity Macaques Pharmacokinetics
This work was supported by the UAB Small Animal Imaging Shared Facility NIH Research Core Grant (P30CA013148); NIH grants R21CA179171, T32CA091078; and an equipment loan from Novadaq and LI-COR Biosciences. The authors would like to thank Dr. Anna Sorace and Ms. Esther de Boer for assistance in this study.
Conflict of Interest
The authors declare no conflict of interest.
- 10.(2004) European public assessment report for Erbitux. Canary Wharf, London UK: European Medicines AgencyGoogle Scholar
- 14.Alberts BJA, Lewis J (2002) Molecular biology of the cell. Garland Science, New YorkGoogle Scholar
- 15.Ross MHKG, Pawlina W (2003) Histology: a text and atlas. Lippincott Williams & Wilkins, New YorkGoogle Scholar
- 16.Spelke E (1994) Anatomy and physiology: an easy learner. Jones and Bartlett Publishers Inc., BurlingtonGoogle Scholar
- 17.En M (1989) Human anatomy and physiology. Benjamin/Cummings Publishing Company Inc., San FranciscoGoogle Scholar