Molecular Imaging and Biology

, Volume 16, Issue 6, pp 846–853

Dose-Dependent Uptake of 3′-deoxy-3′-[18 F]Fluorothymidine by the Bowel after Total-Body Irradiation

  • Markus Hartenbach
  • Andreas Delker
  • Sabrina Hartenbach
  • Juli Schlichtiger
  • Sabrina Niedermoser
  • Carmen Wängler
  • Björn Wängler
  • Guido Böning
  • Franz Josef Gildehaus
  • Klement Neumaier
  • Kirsten Lauber
  • Klaus Kraft
  • Claus Belka
  • Marcus Hacker
  • Viktor Meineke
  • Peter Bartenstein
Research Article

DOI: 10.1007/s11307-014-0755-y

Cite this article as:
Hartenbach, M., Delker, A., Hartenbach, S. et al. Mol Imaging Biol (2014) 16: 846. doi:10.1007/s11307-014-0755-y
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Abstract

Purpose

The aim of this study is to non-invasively assess early, irradiation-induced normal tissue alterations via metabolic imaging with 3′-deoxy-3′-[18 F]fluorothymidine ([18 F]FLT).

Procedures

Twenty-nine male C57BL/6 mice were investigated by [18 F]FLT positron emission tomography for 7 days after total body irradiation (1, 4, and 8 Gy) versus ‘sham’ irradiation (0 Gy). Target/background ratios were determined. The imaging results were validated by histology and immunohistochemistry (Thymidine kinase 1, Ki-67).

Results

[18 F]FLT demonstrated a dose-dependent intestinal accumulation post irradiation. Mean target/background ratio (±standard error) 0 Gy: 1.4 (0.2), 1 Gy: 1.7 (0.1), 4 Gy: 3.1 (0.3), 8 Gy: 4.2 (0.6). Receiver operating characteristic analysis (area under the curve, p value): 0 vs. 1 Gy: 0.81, 0.049; 0 vs. 4 Gy: 1.0, 0.0016; and 0 vs. 8 Gy: 1.0, 0.0020. Immunohistochemistry confirmed the results.

Conclusions

[18 F]FLT seems to provide dose-dependent information on radiation-induced proliferation in the bowel. This opens the perspective for monitoring therapy-related side-effects as well as assessing, e.g., radiation accident victims.

Key words

[18 F]FLT Total body irradiation Bowel Proliferation 

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2014

Authors and Affiliations

  • Markus Hartenbach
    • 1
    • 5
  • Andreas Delker
    • 2
  • Sabrina Hartenbach
    • 3
  • Juli Schlichtiger
    • 2
  • Sabrina Niedermoser
    • 2
    • 7
  • Carmen Wängler
    • 2
    • 8
  • Björn Wängler
    • 2
    • 7
  • Guido Böning
    • 2
  • Franz Josef Gildehaus
    • 2
  • Klement Neumaier
    • 4
  • Kirsten Lauber
    • 4
  • Klaus Kraft
    • 3
  • Claus Belka
    • 4
  • Marcus Hacker
    • 5
  • Viktor Meineke
    • 6
  • Peter Bartenstein
    • 2
  1. 1.Armed Forces HospitalUlmGermany
  2. 2.Department of Nuclear MedicineUniversity Hospital, LMU MunichMunichGermany
  3. 3.Department of PathologyArmed Forces HospitalUlmGermany
  4. 4.Clinic for Radiotherapy and Radiation OncologyUniversity Hospital, LMU MunichMunichGermany
  5. 5.Division of Nuclear Medicine, Department of RadiologyMUWViennaAustria
  6. 6.Armed Forces Institute of RadiobiologyMunichGermany
  7. 7.Molecular Imaging and Radiochemistry, Department of Clinical Radiology and Nuclear MedicineMedical Faculty Mannheim of Heidelberg UniversityMannheimGermany
  8. 8.Biomedical Chemistry, Department of Clinical Radiology and Nuclear MedicineMedical Faculty Mannheim of Heidelberg UniversityMannheimGermany

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