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Comparison of PET Imaging with 64Cu-Liposomes and 18F-FDG in the 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Hamster Buccal Pouch Model of Oral Dysplasia and Squamous Cell Carcinoma

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Abstract

Purpose

Currently, 2-deoxy-2-[18F]fluoro-d-glucose (18F-FDG) is the gold standard radiotracer for staging of head and neck cancer; however, the low sensitivity of this tracer can impede detection of early lesions. 64Cu-liposomes accumulate in various cancers and provide both a sensitive tracer and an indication of the biodistribution of nanotherapeutics. Here, the accumulation of 64Cu-liposomes in early and established cancers is assessed and compared with 18F-FDG in a head and neck cancer model.

Methods

Lesions ranging from mild dysplasia to squamous cell carcinoma were induced in a hamster model of head and neck cancer by topical application of 7,12-dimethylbenz[a]anthracene to the buccal pouch. The hamsters were imaged with micro-positron emission tomography using 18F-FDG and 64Cu-liposomes.

Results

At 24 h postinjection, 64Cu-liposome accumulation exceeded the accumulation of 18F-FDG in every pathologic grade. The lesion-to-cheek pouch (background) ratio and lesion-to-brain ratio were also higher for 64Cu-liposomes than for 18F-FDG.

Conclusion

Imaging of a nanotracer such as 64Cu-liposomes can improve the visualization of head and neck tumors. Accumulation of liposomal particles in head and neck tumors over various pathologic grades averaged 3.5 %ID/cc demonstrating the potential for liposomal therapy with targeted chemotherapeutic agents.

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Acknowledgments

Funding was provided by NIHR01CA103828 and NIHR01CA134659.

Conflict of Interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, University of California, Davis, 451 Health Sciences Drive, Davis, CA, 95616, USA

    Lisa M. Mahakian, Hua Zhang, Jai Woong Seo, Eric M. Haynam & Katherine W. Ferrara

  2. Department of Otolaryngology-Head and Neck Surgery, University of California, Davis, 2521 Stockton Blvd Suite 7200, Sacramento, CA, 95817, USA

    D. Gregory Farwell & David Shaye

  3. Department of Medical Pathology and Laboratory Medicine, University of California, Davis, 4400 V Street, Sacramento, CA, 95817, USA

    Brian Poirier & Alaa M. Afify

  4. Department of Otolaryngology-Research Laboratories, University of California, Davis, 1515 Newton Court Room 211, Davis, CA, 95618, USA

    Steven P. Tinling

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  1. Lisa M. Mahakian
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  2. D. Gregory Farwell
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  3. Hua Zhang
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  4. Jai Woong Seo
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  10. Katherine W. Ferrara
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Corresponding author

Correspondence to Katherine W. Ferrara.

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Mahakian, L.M., Farwell, D.G., Zhang, H. et al. Comparison of PET Imaging with 64Cu-Liposomes and 18F-FDG in the 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Hamster Buccal Pouch Model of Oral Dysplasia and Squamous Cell Carcinoma. Mol Imaging Biol 16, 284–292 (2014). https://doi.org/10.1007/s11307-013-0676-1

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