PET Imaging of Stroke-Induced Neuroinflammation in Mice Using [18F]PBR06
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The purpose of this study is to evaluate the 18 kDa translocator protein (TSPO) radioligand [18F]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([18F]PBR06) as a positron emission tomography (PET) imaging biomarker of stroke-induced neuroinflammation in a rodent model.
Stroke was induced by transient middle cerebral artery occlusion in Balb/c mice. Dynamic PET/CT imaging with displacement and preblocking using PK111195 was performed 3 days later. PET data were correlated with immunohistochemistry (IHC) for the activated microglial markers TSPO and CD68 and with autoradiography.
[18F]PBR06 accumulation peaked within the first 5 min postinjection, then decreased gradually, remaining significantly higher in infarct compared to noninfarct regions. Displacement or preblocking with PK11195 eliminated the difference in [18F]PBR06 uptake between infarct and noninfarct regions. Autoradiography and IHC correlated well spatially with uptake on PET.
[18F]PBR06 PET specifically images TSPO in microglial neuroinflammation in a mouse model of stroke and shows promise for imaging and monitoring microglial activation/neuroinflammation in other disease models.
Key wordsStroke Neuroinflammation Translocator protein 18 kDa (TSPO) Peripheral benzodiazepine receptor (PBR) Positron emission tomography (PET) [18F]PBR06
- 22.Habte F RG, Doyle T, Cheng Z, Gambhir S, Paik D (2011) High-throughput multiple mice imaging on microPET and microPET-CT scanners: evaluation on image quantitation effect. World Molecular Imaging Congress, San DiegoGoogle Scholar