Molecular Imaging and Biology

, Volume 15, Issue 5, pp 576–584

Voxel-Based Analysis of Amyloid-Burden Measured with [11C]PiB PET in a Double Transgenic Mouse Model of Alzheimer’s Disease

  • Boris von Reutern
  • Barbara Grünecker
  • Behrooz H. Yousefi
  • Gjermund Henriksen
  • Michael Czisch
  • Alexander Drzezga
Research Article

Abstract

Purpose

The purpose of this study is to validate the feasibility of a voxel-based analysis of in vivo amyloid-β positron emission tomography (PET) imaging studies in transgenic mouse models of Alzheimer’s disease.

Procedures

We performed [11C]PiB PET imaging in 20 APP/PS1 mice and 16 age-matched controls, and histologically determined the individual amyloid-β plaque load. Using SPM software, we performed a voxel-based group comparison plus a regression analysis between PiB retention and actual plaque load, both thresholded at pFWE < 0.05. In addition, we carried out an individual ROI analysis in every animal.

Results

The automated voxel-based group comparison allowed us to identify voxels with significantly increased PiB retention in the cortical and hippocampal regions in transgenic animals compared to controls. The voxel-based regression analysis revealed a significant association between this signal increase and the actual cerebral plaque load. The validity of these results was corroborated by the individual ROI-based analysis.

Conclusions

Voxel-based analysis of in vivo amyloid-β PET imaging studies in mouse models of Alzheimer’s disease is feasible and allows studying the PiB retention patterns in whole brain maps. Furthermore, the selected approach in our study also allowed us to establish a quantitative relation between tracer retention and actual plaque pathology in the brain in a voxel-wise manner.

Key words

Alzheimer’s disease Double transgenic Mouse model Positron emission tomography Pittsburgh compound B Magnetic resonance imaging Voxel-based Statistical parametric mapping Amyloid precursor protein Presenilin 1 

Abbreviations

PET

Positron emission tomography

AD

Alzheimer’s disease

PiB

Pittsburgh compound B, [11C]-6-OH-BTA-1

Amyloid-β

ROI

Region of interest

SPM

Statistical parametric mapping

tg

Transgenic

wt

Wild type

MRI

Magnetic resonance imaging

APP

Amyloid precursor protein

PS1

Presenilin 1

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Copyright information

© World Molecular Imaging Society 2013

Authors and Affiliations

  • Boris von Reutern
    • 1
    • 2
  • Barbara Grünecker
    • 3
  • Behrooz H. Yousefi
    • 2
    • 4
  • Gjermund Henriksen
    • 2
  • Michael Czisch
    • 3
  • Alexander Drzezga
    • 2
    • 5
  1. 1.Institute of Neuroscience and Medicine (INM-3)Research Centre JülichJülichGermany
  2. 2.Department of Nuclear Medicine, Klinikum rechts der IsarUniversity of Technology MunichMunichGermany
  3. 3.NeuroimagingMax Planck Institute of PsychiatryMunichGermany
  4. 4.Department of Pharmaceutical Radiochemistry, Faculties of Chemistry and MedicineUniversity of Technology MunichGarchingGermany
  5. 5.Department of Nuclear MedicineUniversity Hospital of CologneCologneGermany

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