Molecular Imaging and Biology

, Volume 15, Issue 2, pp 194–202

Molecular Imaging of Neuroblastoma Progression in TH-MYCN Transgenic Mice

  • Carmelo Quarta
  • Erika Cantelli
  • Cristina Nanni
  • Valentina Ambrosini
  • Daniela D’ambrosio
  • Korinne Di Leo
  • Silvia Angelucci
  • Federico Zagni
  • Filippo Lodi
  • Mario Marengo
  • William A. Weiss
  • Andrea Pession
  • Roberto Tonelli
  • Stefano Fanti
Research Article
  • 394 Downloads

Abstract

Purpose

TH-MYCN transgenic mice represent a valuable preclinical model of neuroblastoma. Current methods to study tumor progression in these mice are inaccurate or invasive, limiting the potential of this murine model. The aim of our study was to assess the potential of small animal positron emission tomography (SA-PET) to study neuroblastoma progression in TH-MYCN mice.

Procedure

Serial SA-PET scans using the tracer 2-deoxy-2-[18F]fluoro-d-glucose (18F-FDG) have been performed in TH-MYCN mice. Image analysis of tumor progression has been compared with ex vivo evaluation of tumor volumes and histological features.

Results

[18F]FDG-SA-PET allowed to detect early staged tumors in almost 100 % of TH-MYCN mice positive for disease. Image analysis of tumor evolution reflected the modifications of the tumor volume, histological features, and malignancy during disease progression. Image analysis of TH-MYCN mice undergoing chemotherapy treatment against neuroblastoma provided information on drug-induced alterations in tumor metabolic activity.

Conclusions

These data show for the first time that [18F]FDG-SA-PET is a useful tool to study neuroblastoma presence and progression in TH-MYCN transgenic mice.

Key words

Neuroblastoma Small animal PET [18F]FDG TH-MYCN mice 

Supplementary material

11307_2012_576_MOESM1_ESM.pdf (287 kb)
ESM 1(PDF 286 kb)

Copyright information

© World Molecular Imaging Society 2012

Authors and Affiliations

  • Carmelo Quarta
    • 1
  • Erika Cantelli
    • 2
  • Cristina Nanni
    • 1
  • Valentina Ambrosini
    • 1
  • Daniela D’ambrosio
    • 1
    • 3
  • Korinne Di Leo
    • 2
  • Silvia Angelucci
    • 5
  • Federico Zagni
    • 1
    • 3
  • Filippo Lodi
    • 1
  • Mario Marengo
    • 1
    • 3
  • William A. Weiss
    • 4
  • Andrea Pession
    • 2
  • Roberto Tonelli
    • 5
  • Stefano Fanti
    • 1
  1. 1.Department of Nuclear Medicine, S. Orsola-Malpighi HospitalUniversity of BolognaBolognaItaly
  2. 2.Pediatric Hematology–OncologyUniversity of BolognaBolognaItaly
  3. 3.Department of Medical PhysicsS.Orsola-Malpighi HospitalBolognaItaly
  4. 4.University of California San FranciscoSan FranciscoUSA
  5. 5.Department of PharmacologyUniversity of BolognaBolognaItaly

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