Molecular Imaging and Biology

, Volume 14, Issue 5, pp 541–545

Approach to Assessing Myocardial Perfusion in Rats Using Static [13N]-Ammonia Images and a Small-Animal PET

  • Juan José Vaquero
  • Dong-Wei Gao
  • Carmen García-Villaba
  • Stephen Bacharach
  • Henry VanBrocklin
  • Qizhi Fang
  • Manuel Desco
  • Randall Lee
  • Michael Dae
Brief Article

Abstract

Purpose

Semi-quantitative, static positron emission tomography (PET) has been used to perform an initial approach to the assessment of [13N]-ammonia perfusion studies aimed to elucidating the effect of injecting human embryonic stem cell-derived (hES) hemangioblasts on infarcted rat hearts.

Procedures

Female NIH nude rats underwent occlusion of the left anterior descending coronary artery for 30 min before reperfusion. Either one million hES-derived hemangioblasts (n = 5) or control media (n = 4) were injected into the site of the infarct 1 day post-myocardial infarction (MI) under high-resolution echocardiography guidance. PET imaging was performed 6 weeks after MI induction, and uptake polar maps were created by sampling the left ventricle at equidistant slices from the base to the apex and measuring the average myocardium value at three contiguous voxels to minimize partial volume effects. Statistical comparison between treatment and control groups was done with a Mann–Whitney U test.

Results

Myocardium uptake ratios for treated and untreated subjects show statistically significant difference (98% certainty).

Conclusions

The straightforward procedure described here (similar to those commonly used in clinical routine) was sufficient to yield statistically significant perfusion differences between the treated and untreated animals despite the small sample size.

Key words

Small-animal PET Myocardial perfusion hES hemangioblasts [13N]-Ammonia 

Copyright information

© World Molecular Imaging Society 2012

Authors and Affiliations

  • Juan José Vaquero
    • 1
  • Dong-Wei Gao
    • 2
  • Carmen García-Villaba
    • 3
  • Stephen Bacharach
    • 2
  • Henry VanBrocklin
    • 2
  • Qizhi Fang
    • 2
  • Manuel Desco
    • 1
    • 3
  • Randall Lee
    • 2
  • Michael Dae
    • 2
  1. 1.Departamento de Bioingeniería e Ingeniería AeroespacialUniversidad Carlos III de MadridLeganésSpain
  2. 2.Center for Molecular and Functional ImagingUniversity of California San FranciscoSan FranciscoUSA
  3. 3.Unidad de Medicina y Cirugía ExperimentalHospital General Universitario Gregorio MarañónMadridSpain

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