Molecular Imaging and Biology

, Volume 13, Issue 5, pp 995–1002

Preclinical Imaging of Therapy Response Using Metabolic and Apoptosis Molecular Imaging

  • Marijke De Saint-Hubert
  • Huijun Wang
  • Ellen Devos
  • Kathleen Vunckx
  • Lin Zhou
  • Chris Reutelingsperger
  • Alfons Verbruggen
  • Luc Mortelmans
  • Yicheng Ni
  • Felix M. Mottaghy
Research Article

DOI: 10.1007/s11307-010-0412-z

Cite this article as:
De Saint-Hubert, M., Wang, H., Devos, E. et al. Mol Imaging Biol (2011) 13: 995. doi:10.1007/s11307-010-0412-z

Abstract

Purpose

Early after therapy, 2-deoxy-2-[18F]fluoro-d-glucose ([18F]FDG) imaging is not always reliable due to the influx of inflammatory cells while apoptosis imaging offers a direct and early measurement of therapy effects. This study uses an improved apoptosis probe (99mTc-hAnxA5) in combination with [18F]FDG imaging to evaluate therapy response.

Procedures

Daudi tumor tissue was implanted in the spleen of SCID mice. Treatment was performed with adriamycin and cyclophosphamide. Sequential [18F]FDG-positron emission tomography (PET) was acquired over 6 days and 99mTc-hAnxA5-SPECT was performed before and 1 day after therapy.

Results

On day 1, therapy induced apoptosis was visualized with 99mTc-hAnxA5 without a measurable change in [18F]FDG uptake. [18F]FDG uptake decreased significantly on day 3 and was even more pronounced on day 6.

Conclusion

In this preclinical model, 99mTc-hAnxA5 imaging was able to detect apoptosis before metabolic changes were measured. These results confirm the value of apoptosis imaging for therapy response and give more insight in [18F]FDG imaging and its parameters to evaluate response.

Key words

Lymphoma Chemotherapy response MRI [18F]FDG and 99mTc-hAnxA5 

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010

Authors and Affiliations

  • Marijke De Saint-Hubert
    • 1
  • Huijun Wang
    • 2
  • Ellen Devos
    • 1
  • Kathleen Vunckx
    • 1
  • Lin Zhou
    • 1
  • Chris Reutelingsperger
    • 4
  • Alfons Verbruggen
    • 3
  • Luc Mortelmans
    • 1
  • Yicheng Ni
    • 2
  • Felix M. Mottaghy
    • 1
    • 5
    • 6
  1. 1.Department of Nuclear MedicineUniversity Hospital Gasthuisberg LeuvenLeuvenBelgium
  2. 2.Department of RadiologyUniversity Hospital Gasthuisberg LeuvenLeuvenBelgium
  3. 3.Laboratory for Radiopharmacy, K.U. Leuven, O&N 2LeuvenBelgium
  4. 4.Department of BiochemistryUniversity MaastrichtMaastrichtNetherlands
  5. 5.Klinik für NuklearmedizinUniversitätsklinikum der RWTH AachenAachenGermany
  6. 6.Department of Nuclear MedicineMaastricht University Medical CentreMaastrichtThe Netherlands

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