Molecular Imaging and Biology

, Volume 13, Issue 4, pp 712–720

Localization of Deoxyglucose and Annexin A5 in Experimental Atheroma Correlates with Macrophage Infiltration but not Lipid Deposition in the Lesion

  • Yan Zhao
  • Songji Zhao
  • Yuji Kuge
  • William H. Strauss
  • Francis G. Blankenberg
  • Nagara Tamaki
Research Article

Abstract

Purpose

The aim of this study was to understand the relationship of lipid deposition to the macrophage content, macrophage metabolism, and apoptosis in plaque. We compared the uptake of 2-deoxy-2-fluoro-D-[14C]glucose ([14C]FDG) and [99mTc]HYNIC-annexin V ([99mTc]annexin A5) with the lesion histology in apolipoprotein E knockout (apoE−/−) mice.

Procedures

Male apoE−/− mice (n = 9) were injected with [14C]FDG and [99mTc]annexin A5. Cryostat sections of aorta samples (n = 49) were used for dual-tracer autoradiography, and regional tracer uptake levels were evaluated. Lesions were identified histologically with Movat's pentachrome (AHA lesion phenotypes), Mac-2 staining (macrophage infiltration) and Oil Red O staining (lipid deposition).

Results

The highest uptakes of [14C]FDG (3.10 ± 1.50 %ID × kilogram per square millimeter) and [99mTc]annexin A5 (0.49 ± 0.20 %ID × kilogram per square millimeter) were shown in atheromatous lesions (types III and IV). Each tracer uptake showed better correlation with macrophage infiltration than lipid deposition ([14C]FDG, r = 0.44 vs. r = 0.14; [99mTc]annexin A5, r = 0.65 vs. r = 0.48).

Conclusions

Both tracers were concentrated in type III and IV atheromatous lesions which corresponded to macrophage infiltration rather than lipid deposition.

Key words

Atherosclerosis Imaging Nuclear medicine FDG Annexin A5 

Abbreviations

FDG

2-Deoxy-2-fluoro-D-glucose

HYNIC

Hydrazinonicotinamide

[99mTc]annexin A5

[99mTc]HYNIC-annexin A5

apoE−/− mice

Apolipoprotein E knockout mice

PET

Positron emission tomography

CT

Computed tomography

MRI

Magnetic resonance image

TIA

Transient ischemic attack

ARG

Autoradiography

ROI

Region of interest

ACS

Acute coronary syndrome

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010

Authors and Affiliations

  • Yan Zhao
    • 1
  • Songji Zhao
    • 2
  • Yuji Kuge
    • 3
  • William H. Strauss
    • 4
  • Francis G. Blankenberg
    • 5
  • Nagara Tamaki
    • 1
  1. 1.Department of Nuclear Medicine, Graduate School of MedicineHokkaido UniversitySapporoJapan
  2. 2.Department of Tracer Kinetics and Bioanalysis, Graduate School of MedicineHokkaido UniversitySapporoJapan
  3. 3.Central Institute of Isotope ScienceHokkaido UniversitySapporoJapan
  4. 4.Department of Nuclear MedicineMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  5. 5.Department of Pediatric RadiologyStanford University School of MedicinePalo AltoUSA

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