Molecular Imaging Using Light-Absorbing Imaging Agents and a Clinical Optical Breast Imaging System—a Phantom Study
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The aim of the study was to determine the feasibility of using a clinical optical breast scanner with molecular imaging strategies based on modulating light transmission.
Different concentrations of single-walled carbon nanotubes (SWNT; 0.8–20.0 nM) and black hole quencher-3 (BHQ-3; 2.0–32.0 µM) were studied in specifically designed phantoms (200–1,570 mm3) with a clinical optical breast scanner using four wavelengths. Each phantom was placed in the scanner tank filled with optical matching medium. Background scans were compared to absorption scans, and reproducibility was assessed.
All SWNT phantoms were detected at four wavelengths, with best results at 684 nm. Higher concentrations (≥8.0 µM) were needed for BHQ-3 detection, with the largest contrast at 684 nm. The optical absorption signal was dependent on phantom size and concentration. Reproducibility was excellent (intraclass correlation 0.93–0.98).
Nanomolar concentrations of SWNT and micromolar concentrations of BHQ-3 in phantoms were reproducibly detected, showing the potential of light absorbers, with appropriate targeting ligands, as molecular imaging agents for clinical optical breast imaging.
Key wordsOptical imaging Molecular imaging Breast Transmission
This work was supported, in part, by funding from ART Advanced Research Technologies Inc., National Cancer Institute (NCI) In Vivo Cellular Molecular Imaging Center (ICMIC) grant P50 CA114747 (SSG), NCI Center of Cancer Nanotechnology Excellence (CCNE) CA119367 U54, and the Canary Foundation. We also thank Adam de la Zerda for helpful discussions.
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