Replacing Vascular Corrosion Casting by In Vivo Micro-CT Imaging for Building 3D Cardiovascular Models in Mice
The purpose of this study was to investigate if in vivo micro-computed tomography (CT) is a reliable alternative to micro-CT scanning of a vascular corrosion cast. This would allow one to study the early development of cardiovascular diseases.
Datasets using both modalities were acquired, segmented, and used to generate a 3D geometrical model from nine mice. As blood pool contrast agent, Fenestra VC-131 was used. Batson’s No. 17 was used as casting agent. Computational fluid dynamics simulations were performed on both datasets to quantify the difference in wall shear stress (WSS).
Aortic arch diameters show 30% to 40% difference between the Fenestra VC-131 and the casted dataset. The aortic arch bifurcation angles show less than 20% difference between both datasets. Numerically computed WSS showed a 28% difference between both datasets.
Our results indicate that in vivo micro-CT imaging can provide an excellent alternative for vascular corrosion casting. This enables follow-up studies.
Key wordsFenestra In vivo Micro-CT Mice Vascular corrosion casting 3D model Wall shear stress
Research funded in part by a PhD grant to Bram Trachet of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen). We would like to thank Philippe Joye and Steven Deleye for their help with the animal experiments.
- 4.Dietz HC, Loeys B, Carta L, Ramirez F (2005) Recent progress towards a molecular understanding of Marfan syndrome. Am J Med Genet C Semin Med Genet 139:4–9Google Scholar
- 18.Hodde KC, Miodoński A, Bakker C, Veltman WAM (1977) Scanning electron microscopy of microcorrosion casts with special attention on the arterio-venous differences and application to the rat’s cochlea. Scan Electron Microsc 2:477–484Google Scholar
- 19.Sambach SJ, Bag B, Groden C, Schilling L, Brockmann MA (2010) Vascular imaging in small rodents using micro-CT. Methods 50:26–35Google Scholar
- 21.Casteleyn C, Trachet B, Van Loo D et al (2010) Validation of the murine aortic arch as a model to study human vascular diseases. J Anat 216:563–571Google Scholar
- 23.Hodde KC (1981) Cephalic vascular patterns in the rat: a scanning electron microscopic (SEM) study of casts. Rodopi, Amsterdam, pp 14–15, 32–33, 66Google Scholar