Antioxidants Improve Early Survival of Cardiomyoblasts After Transplantation to the Myocardium
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We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium.
Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI.
Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals.
Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.
Key wordsBioluminescence Firefly luciferase Myoblasts Molecular imaging Stem cell Oxidative stress Myocardium Antioxidants
This work was supported in part by National Health Lung Blood Institute R01 HL078632 (SSG), National Cancer Institute ICMIC CA114747 P50 (SSG), National Institutes of Health K99-R00 HL88048 (MR-P), and the Mayo Clinical Scholarship Program, Mayo Clinic College of Medicine, Rochester, Minnesota (MR-P).
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