Molecular Imaging and Biology

, Volume 12, Issue 3, pp 325–334 | Cite as

Antioxidants Improve Early Survival of Cardiomyoblasts After Transplantation to the Myocardium

  • Martin Rodriguez-Porcel
  • Olivier Gheysens
  • Ramasamy Paulmurugan
  • Ian Y. Chen
  • Karen M. Peterson
  • Jürgen K. Willmann
  • Joseph C. Wu
  • Xiangyang Zhu
  • Lilach O. Lerman
  • Sanjiv S. Gambhir
Research Article

Abstract

Purpose

We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium.

Procedures

Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI.

Results

Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals.

Conclusion

Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.

Key words

Bioluminescence Firefly luciferase Myoblasts Molecular imaging Stem cell Oxidative stress Myocardium Antioxidants 

Notes

Funding

This work was supported in part by National Health Lung Blood Institute R01 HL078632 (SSG), National Cancer Institute ICMIC CA114747 P50 (SSG), National Institutes of Health K99-R00 HL88048 (MR-P), and the Mayo Clinical Scholarship Program, Mayo Clinic College of Medicine, Rochester, Minnesota (MR-P).

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Copyright information

© Academy of Molecular Imaging 2009

Authors and Affiliations

  • Martin Rodriguez-Porcel
    • 1
  • Olivier Gheysens
    • 2
  • Ramasamy Paulmurugan
    • 3
  • Ian Y. Chen
    • 3
    • 4
  • Karen M. Peterson
    • 1
  • Jürgen K. Willmann
    • 3
  • Joseph C. Wu
    • 3
  • Xiangyang Zhu
    • 5
  • Lilach O. Lerman
    • 5
  • Sanjiv S. Gambhir
    • 3
    • 4
  1. 1.Division of Cardiovascular Diseases, Department of Internal MedicineMayo Clinic College of MedicineRochesterUSA
  2. 2.Department of Nuclear MedicineUniversity Hospital LeuvenLeuvenBelgium
  3. 3.Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Division of Nuclear MedicineStanford UniversityStanfordUSA
  4. 4.Department of BioengineeringStanford UniversityStanfordUSA
  5. 5.Division of Nephrology and Hypertension, Department of Internal MedicineMayo Clinic College of MedicineRochesterUSA

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