Molecular Imaging and Biology

, Volume 8, Issue 1, pp 16–23

Combined I-124 Positron Emission Tomography/Computed Tomography Imaging of NIS Gene Expression in Animal Models of Stably Transfected and Intravenously Transfected Tumor

  • David Dingli
  • Brad J. Kemp
  • Michael K. O’Connor
  • John C. Morris
  • Stephen J. Russell
  • Val J. Lowe
Original Article

Abstract

Purpose

With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of I-124 positron emission tomography (PET)/computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to I-123 gamma camera imaging.

Procedures

CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (I-123) and PET/CT (I-124 ) and image quality assessed.

Results

NIS expressing tumors concentrated 7.1% of the injected activity while tumors infected with the control virus had only 0.3% of the activity injected.

Conclusions

I-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.

Key words

Sodium iodide symporter Isotopes Measles virus Virotherapy Oncolysis Molecular imaging Positron emission tomography Computerized tomography Gamma camera 

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Copyright information

© Academy of Molecular Imaging 2005

Authors and Affiliations

  • David Dingli
    • 1
    • 2
  • Brad J. Kemp
    • 3
  • Michael K. O’Connor
    • 3
  • John C. Morris
    • 4
  • Stephen J. Russell
    • 1
    • 2
  • Val J. Lowe
    • 3
  1. 1.Molecular Medicine ProgramMayo Clinic College of MedicineRochesterUSA
  2. 2.Division of HematologyMayo Clinic College of MedicineRochesterUSA
  3. 3.Department of RadiologyMayo Clinic College of MedicineRochesterUSA
  4. 4.Division of Endocrinology and MetabolismMayo Clinic College of MedicineRochesterUSA

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