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Metabolomics

, 15:155 | Cite as

An untargeted metabolomic approach reveals significant postharvest alterations in vitamin metabolism in response to LED irradiation in pak-choi (Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee)

  • Fuhui Zhou
  • Jinhua Zuo
  • Lipu Gao
  • Yuan Sui
  • Qing WangEmail author
  • Aili JiangEmail author
  • Junyan Shi
Original Article

Abstract

The main objective of this study was to investigate the effect of low-level light emitting diode (LED) irradiation on the metabolite profile of pak-choi. A total of 633 different molecular features (MFs) were identified among sample groups (initial, dark-treated, light-treated) using an untargeted metabolomic approach. The identified metabolites were associated with 24 different metabolic pathways. Four of the pathways including carbon pool by folate, folate biosynthesis, thiamine metabolism, and glutathione metabolism, all of which are associated with vitamin biosynthesis, changed significantly. Metabolites in four of the pathways exhibited significant differences from the control in response to LED irradiation. Additionally, porphyrin and chlorophyll metabolism, as well as glucosinolate biosynthesis, riboflavin metabolism, and carotenoid biosynthesis were positively induced by LED irradiation. These results indicate that postharvest LED illumination represents a potential tool for modifying the metabolic profile of pak-choi to maintain quality and nutritional levels.

Keywords

Untargeted metabolomic analysis White LED light irradiation Postharvest quality Vitamin metabolism 

Notes

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2016YFD0400901), the China Agriculture Research System Project (CARS-23), the National Natural Science Foundation of China (31772022), the Natural Science Foundation of Beijing (6182016), Special innovation ability construction fund of Beijing Academy of Agricultural and Forestry Sciences (20180404 and 20180705). We greatly appreciate the critical reading of the manuscript by Dr. Michael Wisniewski, USDA-ARS-Appalachian Fruit Research Station.

Author contributions

QW, AJ, LG designed the experimental trials. FZ and JS performed the experiments, collected the samples. JZ, FZ and YS wrote the article. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal participants

This article does not contain any studies with human participants or animal performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

11306_2019_1617_MOESM1_ESM.xlsx (116 kb)
Supplementary material 1 (XLSX 116 kb)
11306_2019_1617_MOESM2_ESM.xlsx (52 kb)
Supplementary material 2 (XLSX 51 kb)
11306_2019_1617_MOESM3_ESM.docx (659 kb)
Supplementary material 3 (DOCX 659 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) of Ministry of Agriculture, Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijingChina
  2. 2.Key Laboratory of Biotechnology and Bioresources UtilizationMinistry of EducationDalianChina
  3. 3.College of Life ScienceDalian Minzu UniversityDalianChina
  4. 4.Chongqing Key Laboratory of Economic Plant Biotechnology, College of Landscape Architecture and Life Science/Institute of Special PlantsChongqing University of Arts and SciencesChongqingChina

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