Metabolome variability for two Mediterranean sponge species of the genus Haliclona: specificity, time, and space
- 208 Downloads
The study of natural variation of metabolites brings valuable information on the physiological state of the organisms as well as their phenotypic traits. In marine organisms, metabolome variability has mostly been addressed through targeted studies on metabolites of ecological or pharmaceutical interest. However, comparative metabolomics has demonstrated its potential to address the overall and complex metabolic variability of organisms.
In this study, the intraspecific (temporal and spatial) variability of two Mediterranean Haliclona sponges (H. fulva and H. mucosa) was investigated through an untargeted and then targeted metabolomics approach and further compared to their interspecific variability.
Samples of both species were collected monthly during 1 year in the coralligenous habitat of the Northwestern Mediterranean sae at Marseille and Nice. Their metabolomic profiles were obtained by UHPLC-QqToF analyses.
Marked variations were noticed in April and May for both species including a decrease in Shannon’s diversity and concentration in specialized metabolites together with an increase in fatty acids and lyso-PAF like molecules. Spatial variations across different sampling sites could also be observed for both species, however in a lesser extent.
Synchronous metabolic changes possibly triggered by physiological factors like reproduction and/or environmental factors like an increase in the water temperature were highlighted for both Mediterranean Haliclona species inhabiting close habitats but displaying different biosynthetic pathways. Despite significative intraspecific variations, metabolomic variability remains minor when compared to interspecific variations for these congenerous species, therefore suggesting the predominance of genetic information of the holobiont in the observed metabolome.
KeywordsMarine environment Sponges Haliclona Spatio-temporal variability Interspecific variability Specialized metabolites
This project (Grant-Aid Agreement No. PBA/MB/16/01) is carried out with the support of the Marine Institute and is funded under the Marine Research Programme by the Irish Government. M.-A.T. received a Ph.D. scholarship from the French Ministry for Higher education and Research. Metabolomic analyses were performed on the MALLABAR platform (Funded by the CNRS, the Provence Alpes Côte d’Azur Region and the Total Foundation). S. Greff (IMBE Marseille, France) is acknowledged for his help in recording and analysing the metabolomic data.
Methodology and Formal Analysis, M.-A.T., T.P., M.R.; Validation, O.P.T.; Writing—Original Draft Preparation, M.R.; Writing—Review & Editing, O.P.T.; Supervision, T.P., O.P.T.; Project Administration, O.P.T.; Funding Acquisition, T.P., O.P.T.
This project (Grant-Aid Agreement No. PBA/MB/16/01) is carried out with the support of the Marine Institute and is funded under the Marine Research Programme by the Irish Government. The Ph.D. scholarship of M.-A. Tribalat has been funded by the French “Ministère de lʼEnseignement supérieur, de la Recherche et de lʼInnovation”.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.
- Alarif Walied, M., Abdel-Lateff, A., Al-Lihaibi Sultan, S., Seif-Eldin, A., N. & Badria Farid, A. (2013). A new cytotoxic brominated acetylenic hydrocarbon from the marine sponge Haliclona sp. with a selective effect against human breast cancer. Zeitschrift für Naturforschung C, 68, 70–75.CrossRefGoogle Scholar
- Borchert, E., Jackson, S. A., O’gara, F., & Dobson, A. D. W. (2016). Diversity of natural product biosynthetic genes in the microbiome of the deep sea sponges Inflatella pellicula, Poecillastra compressa, and Stelletta normani. Frontiers in Microbiology, 7, 1027.CrossRefPubMedPubMedCentralGoogle Scholar
- Costa-Lotufo, L. V., Carnevale-Neto, F., Trindade-Silva, A. E., Silva, R. R., Silva, G. G. Z., Wilke, D. V., Pinto, F. C. L., Sahm, B. D. B., Jimenez, P. C., Mendonca, J. N., Lotufo, T. M. C., Pessoa, O. D. L., & Lopes, N. P. (2018). Chemical profiling of two congeneric sea mat corals along the Brazilian coast: Adaptive and functional patterns. Chemical Communications, 54, 1952–1955.CrossRefPubMedGoogle Scholar
- De’ath, G. (2002). Multivariate regression trees: A new technique for modeling species-environment relationships. Ecology, 83, 1105–1117.Google Scholar
- Glassmire, A. E., Jeffrey, C. S., Forister, M. L., Parchman, T. L., Nice, C. C., Jahner, J. P., Wilson, J. S., Walla, T. R., Richards, L. A., Smilanich, A. M., Leonard, M. D., Morrison, C. R., Simbaña, W., Salagaje, L. A., Dodson, C. D., Miller, J. S., Tepe, E. J., Villamarin-Cortez, S., & Dyer, L. A. (2016). Intraspecific phytochemical variation shapes community and population structure for specialist caterpillars. New Phytologist, 212, 208–219.CrossRefPubMedGoogle Scholar
- Haas, A. F., Fairoz, M. F. M., Kelly, L. W., Nelson, C. E., Dinsdale, E. A., Edwards, R. A., Giles, S., Hatay, M., Hisakawa, N., Knowles, B., Lim, Y. W., Maughan, H., Pantos, O., Roach, T. N. F., Sanchez, S. E., Silveira, C. B., Sandin, S., Smith, J. E., & Rohwer, F. (2016). Global microbialization of coral reefs. Nature Microbiology, 1, 16042.CrossRefPubMedGoogle Scholar
- Li, D., Baldwin, I. T., & Gaquerel, E. 2015. Navigating natural variation in herbivory-induced secondary metabolism in coyote tobacco populations using MS/MS structural analysis. Proceedings of the National Academy of Sciences, 112, E4147–E4155.Google Scholar
- Pohnert, G. (2004). Chemical defense strategies of marine organisms. In S. SCHULZ (Ed.), The chemistry of pheromones and other semiochemicals I. Berlin: Springer.Google Scholar
- Redmond, N. E., Raleigh, J., Van Soest, R. W. M., Kelly, M., Travers, S. A. A., Bradshaw, B., Vartia, S., Stephens, K. M., & Mccormack, G. P. (2011). Phylogenetic relationships of the marine Haplosclerida (Phylum Porifera) employing ribosomal (28S rRNA) and mitochondrial (cox1, nad1) gene sequence data. PLoS ONE, 6, e24344.CrossRefPubMedPubMedCentralGoogle Scholar
- Schmitt, S., Tsai, P., Bell, J., Fromont, J., Ilan, M., Lindquist, N., Perez, T., Rodrigo, A., SCHUPP, P. J., Vacelet, J., Webster, N., Hentschel, U., & Taylor, M. W. (2012). Assessing the complex sponge microbiota: Core, variable and species-specific bacterial communities in marine sponges. The ISME Journal, 6, 564–576.CrossRefPubMedGoogle Scholar
- Shin, B. A., Kim, Y. R., Lee, I.-S., Sung, C. K., Hong, J., Sim, C. J., Im, K. S., & Jung, J. H. (1999). Lyso-PAF analogues and lysophosphatidylcholines from the marine sponge Spirastrella abata as inhibitors of cholesterol biosynthesis. Journal of Natural Products, 62, 1554–1557.CrossRefPubMedGoogle Scholar
- Stanley, D. W. 2000. Eicosanoids in invertebrate signal transduction systems, Princeton: Princeton University Press.Google Scholar
- Tribalat, M.-A. 2016. Specialized metabolisms of Mediterranean sponges of genus Haliclona Grant, 1836. Nice: Université Côte d’Azur.Google Scholar