Metabolites predict cardiovascular disease events in persons living with HIV: a pilot case–control study
Persons living with HIV (PLWH) are at higher risk for cardiovascular disease (CVD) events than uninfected persons. Current risk-stratification methods to define PLWH at highest risk for CVD events are lacking.
Using tandem flow injection mass spectrometry, we quantified plasma levels of 60 metabolites in 24 matched pairs of PLWH [1:1 with and without known coronary artery disease (CAD)]. Metabolite levels were reduced to interpretable factors using principal components analysis.
Factors derived from short-chain dicarboxylacylcarnitines (SCDA) (p = 0.08) and glutamine/valine (p = 0.003) were elevated in CAD cases compared to controls.
SCDAs and glutamine/valine may be valuable markers of cardiovascular risk among persons living with HIV in the future, pending validation in larger cohorts.
KeywordsCardiovascular disease HIV Metabolomics Acute coronary syndrome Myocardial infarction
This research was funded by the National Heart, Lung and Blood Institute (R56 HL129880) and by the Duke Centers for AIDS Research (P30 AI064518).
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
Research involving human participants and/or animals
Ethical approval for the use of patient clinical data and specimens were approved by the Duke University Institutional Review Board.
Patient consent was obtained at the time of sample collection for the Duke HIV Biorepository with the understanding that their samples would be used for future research studies.
- Friis-Moller, N., Thiebaut, R., Reiss, P., Weber, R., Monforte, A. D., De Wit, S., et al. (2010). Predicting the risk of cardiovascular disease in HIV-infected patients: The data collection on adverse effects of anti-HIV drugs study. European Journal of Preventive Cardiology, 17, 491–501.CrossRefGoogle Scholar
- Newgard, C. B., An, J., Bain, J. R., Muehlbauer, M. J., Stevens, R. D., Lien, L. F., et al. (2009) A branched-chain amino acid-related metabolic signature that differentiates obese and lean humans and contributes to insulin resistance. Cell Metabolism, 9, 311–326.CrossRefPubMedPubMedCentralGoogle Scholar
- Shah, S. H., Bain, J. R., Muehlbauer, M. J., Stevens, R. D., Crosslin, D. R., Haynes, C., et al. (2010) Association of a peripheral blood metabolic profile with coronary artery disease and risk of subsequent cardiovascular events. Circulation: Cardiovascular Genetics, 3, 207–214.Google Scholar